The activities of the MSM department "morphogenesis, signaling, modeling" are dedicated to the study of the mechanisms of plant morphogenesis. Our studies are focused primarily on the cellular level (control of cell division, cell polarity ...) but also extend to the whole plant (eg signaling between organs). We are interested in the issues of totipotency and cell differentiation in the plant but also in vitro. We use modeling approaches to better understand these complex phenomena.
The teams from the MSM department not only work on biological models such as Arabidopsis thaliana, Brachypodium or the moss Physcomitrella patens but also on species of agronomic interest such as pea or cotton. We mainly use classical approaches of genetics and functional genomics complemented by methods of biochemistry and chemistry. We exploit many imaging techniques for which we develop novel quantitative approaches. The purpose of our work is a better understanding of plant development in order to propose innovative strategies to improve plant resources and their uses. This happens, for example by modifying plant architecture, and further upstream, by improving processing techniques and regeneration.
The team « Biology of the Cell and Regeneration » is focused on control of regeneration technics
by organogenesis or somatic embryogenesis and genes transfer
to in vitro grown plant cells. Objectives concern
fundamental uses (transient activities, "knock-out"...)
or more focused studies (resistance to abiotic stresses, biomass
use…). Model plants Arabidopsis thaliana and Brachypodium
distachyon and also agronomical important crops sorghum
and cotton are the studied species. The group is involved
in a project aimed to characterize cell regenerating transcriptome
profiles (organogenesis in Arabidopsis thaliana and
somatic embryogenesis in cotton).
The team « Spatial control of cell division » In higher plants, the cytoskeleton steadily undergoes
structural reorganizations which accompany and/or determine
every stage in cell division, elongation and differentiation.
Our aim is to characterize cellular activities and regulation
networks involved in the organization of microtubule arrays
during the cell cycle in land plants. Our studies are focused
on entry into mitosis and premitotic determination of the
division plane in plant cells. We try to clarify the role
of the preprophase band, a specific feature of plant cells,
and more generally to explore the links between the microtubule
cytoskeleton and the cell cycle machinery. Recent results
of the group have revealed a large regulatory complex (TTP)
composed of several protein partners. The recruitment of the
TTP complex to the cytoskeleton, and its activity at the G2/M
transition are necessary for division plane determination
and PPB formation in Arabidopsis and moss. This complex also
plays a pivotal role in the organization of the interphase
array during diffuse growth.
The team « Branching control in plants » aims to have a better understanding of the genetic, molecular and physiological factors that control axillary buds outgrowth or dormancy. In particular, we focus on the biosynthesis and the signaling pathways of a novel class of phytohormones, the strigolactones, which inhibit branching. Our work is based on a large collection of pea mutants specifically affected in stem branching. The same genes are studied in the moss Physcomitrella patens, to better understand the evolution of strigolactone function in land plants.
The team « Cell differentiation
and polarity » team studies cellular mechanisms
of development associated to the acquisition of cell identity
thought two models: tumor mutants and phloem ontogenesis.
The group is focused on the role of fatty acids on cell differentiation
and polarity, particularly on biosynthesis, compartmentalization
and the role of long chain fatty acids in general and more
precisely sphingolipids. Role of palmitoylation is also studied.
The team « Transcription factors and plant architecture » focuses on the mechanisms controlling the morphogenesis of the aerial part of plants and on their evolution. For this, we study the function of two families of transcription factors (homeodomain proteins of the TALE family proteins and NAM / CUC proteins regulated by the microRNA miR164) in the development of meristems, leaves and flowers in Arabidopsis and other species.
The team « Modeling and Digitial Imaging » develops methods, algorithms and tools for processing, analyzing and modeling data generated, primarily, by biological imaging techniques. We aim, on the one hand, at unraveling principles of spatial organization and, on the other hand, at understanding the factors and mechanisms that subtend these principles. Our activity is thus at the intersection of image processing and analysis, spatial statistics, and modeling.
Selected papers :
Andrey P, Kieu K, Kress C, Lehmann G, Tirichine L, Liu Z, Biot E, Adenot PG, Hue-Beauvais C, Houba-Herin N, Duranthon V, Devinoy E, Beaujean N, Gaudin V, Maurin Y, Debey P (2010) Statistical analysis of 3D images detects regular spatial distributions of centromeres and chromocenters in animal and plant nuclei. PLoS Comput Biol 6: e1000853 (pubmed)
Azimzadeh J, Nacry P, Christodoulidou A, Drevensek S, Camilleri C, Amiour N, Parcy F, Pastuglia M, Bouchez D (2008) Arabidopsis TONNEAU1 proteins are essential for preprophase band formation and interact with centrin. Plant Cell 20: 2146-2159 (pubmed)
Bach L, Gissot L, Marion J, Tellier F, Moreau P, Satiat-Jeunemaitre B, Palauqui JC, Napier JA, Faure JD (2011) Very-long-chain fatty acids are required for cell plate formation during cytokinesis in Arabidopsis thaliana. J Cell Sci 124: 3223-3234 (pubmed)
Bach L, Michaelson LV, Haslam R, Bellec Y, Gissot L, Marion J, Da Costa M, Boutin JP, Miquel M, Tellier F, Domergue F, Markham JE, Beaudoin F, Napier JA, Faure JD (2008) The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development. Proc Natl Acad Sci U S A 105: 14727-14731 (pubmed)
Blein T, Hasson A, Laufs P (2010) Leaf development: what it needs to be complex. Curr Opin Plant Biol 13: 75-82 (pubmed)
Blein T, Pulido A, Vialette-Guiraud A, Nikovics K, Morin H, Hay A, Johansen IE, Tsiantis M, Laufs P (2008) A conserved molecular framework for compound leaf development. Science 322: 1835-1839 (pubmed)
Braun N, de Saint Germain A, Pillot JP, Boutet-Mercey S, Dalmais M, Antoniadi I, Li X, Maia-Grondard A, Le Signor C, Bouteiller N, Luo D, Bendahmane A, Turnbull C, Rameau C (2012) The Pea TCP Transcription Factor PsBRC1 Acts Downstream of Strigolactones to Control Shoot Branching. Plant Physiol 158: 225-238 (pubmed)
Chupeau Y, Davey M (2007) Gene transfer to plants. In Functional Plant Genomics, Enfield, NH, USA Editor : Morot-Gaudry, JF, Lea, P, Briat, JF: 123-142
Drevensek S, Goussot M, Duroc Y, Christodoulidou A, Steyaert S, Schaefer E, Duvernois E, Grandjean O, Vantard M, Bouchez D, Pastuglia M (2012) The Arabidopsis TRM1-TON1 interaction reveals a recruitment network common to plant cortical microtubule arrays and eukaryotic centrosomes. The Plant cell 24: 178-191 (pubmed)
Dun EA, de Saint Germain A, Rameau C, Beveridge CA (2012) Antagonistic action of strigolactone and cytokinin in bud outgrowth control. Plant Physiol 158: 487-498 (pubmed)
Gomez-Roldan V, Fermas S, Brewer PB, Puech-Pages V, Dun EA, Pillot JP, Letisse F, Matusova R, Danoun S, Portais JC, Bouwmeester H, Becard G, Beveridge CA, Rameau C, Rochange SF (2008) Strigolactone inhibition of shoot branching. Nature 455: 189-194 (pubmed)
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P (2011) Evolution and Diverse Roles of the CUP-SHAPED COTYLEDON Genes in Arabidopsis Leaf Development. Plant Cell 23: 54-68 (pubmed)
Konan KE, Durand-Gasselin T, Kouadio YJ, Flori A, Rival A, Duval Y, Pannetier C (2010) In vitro conservation of oil palm somatic embryos for 20 years on a hormone-free culture medium: characteristics of the embryogenic cultures, derived plantlets and adult palms. Plant Cell Rep 29: 1-13 (pubmed)
Markham JE, Molino D, Gissot L, Bellec Y, Hematy K, Marion J, Belcram K, Palauqui JC, Satiat-Jeunemaitre B, Faure JD (2011) Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in Arabidopsis. The Plant cell 23: 2362-2378 (pubmed)
Proust H, Hoffmann B, Xie X, Yoneyama K, Schaefer DG, Nogue F, Rameau C (2011) Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Physcomitrella patens. Development 138: 1531-1539 (pubmed)
Ragni L, Belles-Boix E, Gunl M, Pautot V (2008) Interaction of KNAT6 and KNAT2 with BREVIPEDICELLUS and PENNYWISE in Arabidopsis inflorescences. Plant Cell 20: 888-900 (pubmed)
Roudier F, Gissot L, Beaudoin F, Haslam R, Michaelson L, Marion J, Molino D, Lima A, Bach L, Morin H, Tellier F, Palauqui JC, Bellec Y, Renne C, Miquel M, Dacosta M, Vignard J, Rochat C, Markham JE, Moreau P, Napier J, Faure JD (2010) Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis. Plant Cell 22: 364-375 (pubmed)
Spinner L, Pastuglia M, Belcram K, Pegoraro M, Goussot M, Bouchez D, Schaefer DG (2010) The function of TONNEAU1 in moss reveals ancient mechanisms of division plane specification and cell elongation in land plants. Development 137: 2733-2742 (pubmed)
Tirichine L, Andrey P, Biot E, Maurin Y, Gaudin V (2009) 3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei. Plant Methods 5: 11 (pubmed)
Truernit E, Bauby H, Belcram K, Barthelemy J, Palauqui JC (2012) OCTOPUS, a polarly localised membrane-associated protein, regulates phloem differentiation entry in Arabidopsis thaliana. Development 139: 1306-1315 (pubmed)
Truernit E, Bauby H, Dubreucq B, Grandjean O, Runions J, Barthelemy J, Palauqui JC (2008) High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis. Plant Cell 20: 1494-1503 (pubmed)