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Secondary Cell wall

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Keywords :Arabidopsis thaliana - Soluble phenolic compounds -Lignin -Mutant - Secondary cell wall - Biotic and abiotic stresses - Xylans

Doctoral school affiliation : ED145 "Sciences du Végétal" Université Paris 11-Orsay

Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 2
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19

	Group leader Richard Sibout Research Scientist
	Nathalie Demont-Caulet, Associate Professor Paris VII
	Philippe Le Bris Technician INRA
	Yin Wang PhD Student from 01/09/11 to 31/08/14
	Aurélie Scagnelli Engineer from 19/09/11 to 18/08/12

	Lise Jouanin Senior scientist CNRS
	Sébastien Antelme Technician INRA " Brachypodium "
	Davy Baratiny PhD student départements INRA BV, CEPIA et fondation Tuck from 01/10/10 to 30/09/13
	Marie Séverine Ho Yue Kuang PhD Student départments BV and CEPIA from 01/10 to 30/09/14

Objectives :

Lignified secondary cell walls are primordial for plant growth and development.

coloration des lignines

They allow sap conduction in xylem vessels, are involved in erect growth of plants and in resistance to biotic and abiotic stresses. Understanding the mechanisms of secondary cell wall formation has major scientific and economic perspectives with the use of plant lignocellulosic biomass for biofuels and green chemistry.
Use of mutants in model plants, Arabidopsis thaliana and Brachypodium distachyon, is the strategy chosen to identify new genes involved in biosynthesis and structure of secondary cell wall and mainly in lignification.
The different steps of the lignin pathway involve enzymes encoded by members of multigene families. Arabidopsis mutants for each of the genes that are potentially implicated are being identified from existing mutant collections and characterized.
For Brachypodium, a collection of about 6000 chemically mutated lines has been generated in Versailles. This collection is being used to identify genes involved in secondary cell wall formation using forward genetics and tilling.
The impact of the mutations on the cell wall polymer (polysaccharides and lignins) content and composition is being determined in collaboration with the IJPB green chemistry platform and the « lignin and tannins » team.

Main Results :

Characterization of mutants or antisense plants for genes (COMT, CCoAOMT, F5H, CCR) involved in the first steps of the lignin pathway (monolignol biosynthesis) has demonstrated the interest of Arabidopsis in the study of this metabolic pathway.. The consequences of deregulation of these genes are similar in this model plant to that in plants of agronomical interest such as poplar, a tree used in our previous studies. This work led to the identification, within the multigene families, of the genes specifically involved in the constitutive lignin pathway. In some cases, the absence of expression of only one gene (COMT and F5H) leads to phenotypes, whereas in other cases the mutations of two genes (CAD) are needed to observe an impact on lignin content and composition. The situation is more complex for the lignin polymerisation step where large multigenic families (peroxidases and laccases) are believed to be involved. Recent work has demonstrated the importance of two laccase genes in this step.
In the case of Brachypodium, the grass model plant, orthologs of the identified genes in Arabidopsis have been identified and mutants are currently being characterized.
These research projects are performed in the frame of ANR and European financed programs.

Selected Publications :

Rkljacic et al. (2011). Brachypodium as a model for the grasses: Today and the future. Plant Physiol 157: 3

Sanchez-Rodriguez C, Rubio-Somoza I, Sibout R, Persson S (2010) Phytohormones and the cell wall in Arabidopsis during seedling growth. . Trends Plant Sci. 2010 May;15(5):291-301.

I nternational Brachypodium Initiative. (2010) Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature. 2010 Feb 11;463(7282):763-8.

Quentin M, Allasia V, Pegard A, Allais F, Ducrot P-H, Favery B, Levis C, Martinet S, Masur C, Ponchet M, Roby D, Schlaich N, Jouanin L, Keller H (2009) Imbalanced lignin biosynthesis promotes the sexual reproduction of homothallic oomycete pathogens. PloS Pathogens 5 : 1-9. (PubMed)

Minic Z, Jamet E, San-Clemente H, Peleltier S, Renou J-P, Rihouey C, Okinyo D, Proux C, Lerouge P, Jouanin L (2009) Transcriptomic analysis of Arabidopsis developing stems : a close-up on cell wall genes. BMC Plant Biology, Jan 16;9(1):6.(PubMed)

Ralph J, Kim H, Lu F, Grabber J, Leplé JC, Berrio-Sierra J,(Meyers et al., 2008) Mir Derikvand M, Jouanin L, Boerjan W, Lapierre C (2008) Identification and origin of a thioacidolysis marker compound for ferulic acid incorporation into angiosperm lignins (and a pseudo-marker compound for cinnamoyol-CoA reductase deficiency). Plant J 53 : 368-379. (PubMed)

Mir Derikvand M, Berrio-Sierra J, Ruel K, Pollet B, Do C-T, Thévenin J, Buffard D, Jouanin L, Lapierre C (2008) Redirection of the phenylpropanoid pathway to feroloyl malate in Arabidopsis mutants deficient for cinnamoyl-CoA reductase. Planta 227 : 943-956.(PubMed)

Chavez Montes RA, Ranocha P, Martinez Y, Minic Z, Jouanin L, Marquis M, Saulnier L, Fulton LM, Cobbett CS, Bitton F, Renu JP, Jauneau A, goffner D (2008) Cell wall modifications in Arabidopsis plants with alterned alpha-L- arabinofuranosidae activity. Plant Physiol 147 : 63-77. (PubMed)

Eudes A, Mouille G, Thevenin J, Minic Z, Jouanin L (2008) Purification, cloning and functional characterization of an endogenous beta-glucosidase in Arabidopsis thaliana. Plant Cell Physiol 49 : 1331-1341. (PubMed)

Quentin M, Allasia V, Pegard A, Allais F, Ducrot P-H, Favery B, Levis C, Martinet S, Masur C, Ponchet M, Roby D, Schlaich N, Jouanin L, Keller H (2009) Imbalanced lignin biosynthesis promotes the sexual reproduction of homothallic oomycete pathogens. PloS Pathogens 5 : 1-9. (PubMed)

Minic Z, Jamet E, San-Clemente H, Peleltier S, Renou J-P, Rihouey C, Okinyo D, Proux C, Lerouge P, Jouanin L (2009) Transcriptomic analysis of Arabidopsis developing stems : a close-up on cell wall genes. BMC Plant Biology, Jan 16;9(1):6.(PubMed)

Meyers, B.C., Axtell, M.J., Bartel, B., Bartel, D.P., Baulcombe, D., Bowman, J.L., Cao, X., Carrington, J.C., Chen, X., Green, P.J., Griffiths-Jones, S., Jacobsen, S.E., Mallory, A.C., Martienssen, R.A., Poethig, R.S., Qi, Y., Vaucheret, H., Voinnet, O., Watanabe, Y., Weigel, D., and Zhu, J.K. (2008). Criteria for Annotation of Plant MicroRNAs. Plant Cell.2008 Dec;20(12):3186-90..(PubMed)

Do C-T, Pollet B, Thévenin J, Sibout R, Denoue D, Barrière Y, Lapierre C, Jouanin L.(2007) Both caffeoyl CoA enzyme 3-O-methyltransferase and caffeic acid O-methyltransferase I are involved in redundant functions for lignins, flavonoids and sinapoyl malate biosynthesis in Arabidopsis. Planta, 226, 1117-29 (PubMed)

Minic Z, Jamet E, Négroni L, der Garabedian, Zivy M, Jouanin L (2006) A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases.. J Exp Bot 58 : 2503-2512. (PubMed)

Eudes A, Pollet B, Sibout R, Do C-T, Séguin A, Lapierre C, Jouanin L (2006) Evidence for a role of AtCAD1 in lignification of elongating stems of Arabidopsis thaliana. Planta 225, 23-39. (PubMed)

Minic Z, Jouanin L (2006) Plant glycoside hydrolases in cell wall polysaccharide degradation. Plant Physiol Biochem 44, 435-449. (PubMed)

Minic Z, Do C-T, Rihouey C, Morin H, Lerouge P, Jouanin L (2006) Purification, functional characterization, cloning, and identification of mutants of a seed specific arabinan hydrolase in Arabidopsis. J Exp Bot 57, 2339-2351. (PubMed)

Barrière Y, Denoue D, Briand M, Simon M, Jouanin L, Durand-Tardif M (2006) Genetic variations of cell wall digestibility related traits in floral stems of Arabidopsis thaliana accessions as a basis for the improvement of the feeding value in maize and forage plants. Theor Appl Genet 113, 163-175. (PubMed)

Pineau C, Freydier A, Ranocha P, Jauneau A, Turner S, Lemonnier G, Renou J-P, Tarkowski P, Sandberg G, Jouanin L, Sundberg B, Boudet A-M, Goffner D, Pichon M (2005) hca, an Arabidopsis mutant exhibiting unusual cambial activity and altered vascular patterning. Plant J. 44, 271-289 (PubMed)

Barrière Y, Laperche A, Barrot L, Aurel G, Briand M, Jouanin L (2005) QTL analysis of lignification and cell wall digestibility in the Bay0 x Shahdara RIL progeny of Arabidopsis thaliana as a model system for forage plant. Plant Sci 168: 1235-1245.

Sibout R, Eudes A, Mouille G, Pollet B, Lapierre C, Jouanin L, Séguin A (2005). Cinnamyl alcohol dehydrogense C and D are the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis. Plant Cell 17: 2059-2076. (PubMed)

Minic Z, Rihouey C, Do CT, Lerouge P, Jouanin L (2004) Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis thaliana. Plant Physiol. 135: 867-78 (PubMed)

Lapierre C, Pilate G, Pollet B, Mila I, Leplé J-C, Jouanin L, Kim H, Ralph J (2004) Signatures of cinnamyl alcohol dehydrogenases deficiency in poplar lignins. Phytochemistry 65 : 313-321. (PubMed)

Goujon T, Sibout R, Pollet B, Maba B, Nussaume L, Bechtold N, Lu F, Ralph J, Mila I, Barrière Y, Lapierre C, Jouanin, L (2003) A new Arabidopsis thaliana mutant deficient in the expression of O-methyltransferase 1: Impact on lignins and on sinapoyl esters. Plant Mol Biol 51 : 973-989. (PubMed)

Goujon T, Minic Z, El Amrani A, Lerouxel O, Aletti E, Lapiere C, Joseleau J-P, Jouanin L (2003) A novel higher plant (Arabidopsis thaliana) beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development. Plant J 33 : 677-690. (PubMed)

Goujon T, Ferret V, Mila I, Pollet B, Ruel K, Burlat V, Joseleau J-P, Barrière Y, Lapierre C, Jouanin L (2003) Down-regulation of AtCCR1 gene in Arabidopsis thaliana : effects on phenotype, lignins and cell degradability. Planta 217 : 218-228. (PubMed)

Sibout R, Eudes A, Pollet B, Goujon T, Mila I, Granier F, Séguin A, Lapierre C, Jouanin L (2003) Expression pattern of two paralogues encoding cinnamyl alcohol dehydrogenase in Arabidopsis thaliana: Isolation and characterization of the corresponding mutants. Plant Physiol 132 : 848-860. (PubMed)

Goujon T, Sibout R, Eudes A, Jouanin L (2003) Genes involved in the biosynthesis of lignin precursors in Arabidopsis thaliana. Plant Physiol Biochem 41: 677-687.

Pilate G, Guiney E, Petit-Conil M, Lapierre C, Leplé J-C, Pollet B, Mila I, Halpin C, Jouanin L, Boerjan W, Schuch W, Cornu D (2002) Field performance of transgenic trees with altered lignin metabolism. Nature Biotech 20, 607-612. (PubMed)

Ruel K, Montiel MD, Goujon T, Jouanin L, Burlat V, Joseleau JP (2002) Interaction between lignin deposition and polysaccharide matrices during the assembly of the plant cell wall. Plant Biol 51, 2-8.

Sibout R, Baucher M, Gatineau M, Van Doorsselaere J, Mila I, Pollet B, Maba B, Pilate G, Lapierre C, Boerjan W, Jouanin L (2002) Expression of a poplar cDNA encoding a ferulate-5-hydroxylase increases S lignin deposition in Arabidopsis thaliana. Plant Physiol Biochem, 40, 1087-1096

Ralph J, Lapierre C, Marita J, Kim H, Fachuang L, Hatfield R, Ralph S, Chapple C, Franke R, Hemm M, Mackay J, Yahiaoui N, Boudet A, Pean M, Pilate G, Boerjan W, Jouanin L (2001) Elucidation of new structures in lignins of CAD- and COMT-deficient plants by NMR. PhytoChemistry 57, 99-1003. (PubMed)

Jouanin L, Goujon T, de Nadaï V, Martin MT, Mila I, Vallet C, Pollet B, Yoshinaga A, Chabbert B,Petit-Conil M, Lapierre C (2000) Lignification in transgenic poplars with extremely reduced caffeic acid O-methyltransferase. Plant Physiol 123 : 1363-1374. (PubMed)

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