IJPB-Organelles and reproduction

Organelles and reproduction

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Scientific topics: Plant Genetics, Plant Molecular Biology, Biotechnology, Cell Biology, Developmental Biology, Functional Genomics.lant Genetics, Plant Molecular Biology, Biotechnology, Cell Biology, Developmental Biology, Functional Genomics.

Keywords : Sexual reproduction, Mutant, Mitochondria, Plastids, PPR proteins, Nucleo-cytoplasmic male sterility, Arabidopsis thaliana, Rapeseed (Brassica napus), Raphanus

Doctoral school affiliationt : ED 145 Sciences du végétal, Université Paris-Sud 11, Orsay

Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 7
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 31 80- fax : +33 (0)1 30 83 33 19

	Group leader Françoise Budar Senior scientist Cytonuclear co-adaptation in Arabidopsis thaliana Evolution of restoration genes in the Ogura Cytoplasmic Male Sterility system
	Estelle Wenes Engineer
	Martine Quadrado Technician
	Carine Rizzolatti PhD student
	Jennifer Dahan post-doc ANR from 01/02/10 to 31/01/13

	Hakim Mireau Research scientist Functional and biochemical study of PPR proteins.
	Nadège Arnal Technician
	Nicolas Gobron PhD student
	Nawel Haili PhD student
	Gregory Brown Professor University McGill (Canada) from 01/08/11 to 31/07/12

Summary :

Fluorescence image of an anther from an Arabidopsis thaliana transgenic plant producing a mitochondria-targeted GFP specifically in the tapetum. The red fluorescence is the auto-fluorescence of chlorophyll, the green fluorescence corresponds to GFP.

Our research programs focus mainly on the analysis of the functional relationship existing between the plant cell nucleus and 2 types of cellular organelles, the mitochondria and the chloroplasts. Besides the comprehension of these phenomena at the molecular level, we are interested in understanding the effects of this crosstalk on plant sexual reproduction. Cytoplasmic male sterilities (CMS) have long illustrated the peculiar involvement of mitochondria in plant sexual reproduction. CMSs, which condition the inability to produce functional pollen, are being used in various plant species to facilitate the production of hybrid seeds in self-pollinating crop species. They actually rely on a double genetic determinant: a mitochondrial gene that induces the sterility and a counter-acting nuclear gene restoring the male fertility.
The analysis of nuclear factors regulating the expression of chloroplast and mitochondrial genes is also based on the biochemical characterization of PPR proteins (Pentatricopeptide Repeat). A fair number of PPR proteins have been shown to take part in various aspect of post-transcriptional regulation of gene expression in these organelles. Our interest for this family of proteins is also motivated by the fact that all but one genes restorers of fertility in CMS systems code for PPR proteins.

Main Results :

We contributed to the identification of the Rfo gene, the restorer of fertility in the Ogura CMS system (Desloire et al, 2003). Using biochemical approaches, we showed that the restoring function of the Rfo gene product, a PPR protein, is correlated with its capacity to interact with the sterility gene mRNA (Uyttewaal et al, 2008b). We analyzed the sequence of a non-restoring allele of the Rfo locus and compared the PPR genes of this allele with those of the restorer one. We found that intra- and inter-genic recombinations occurred during the evolution of the Rfo locus (Hernandez Mora et al, 2010).

We also analyzed cytoplasmic diversity among 95 accessions of Arabidopsis thaliana, and built a phylogeny of A. thaliana cytotypes (Moison et al, 2010). Our results also suggest that there is co-adaptation between nuclear and organelle genomes in this species.

Selected Publications :

Hernandez-Mora, J.R., E. Rivals, H. Mireau and F. Budar (2010) « Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo). » BMC Plant Biol 10 :35

Moison M, Roux F, Quadrado M, Duval R, Ekovich M, Lê DH, Verzaux M, Budar F(2010) Cytoplasmic phylogeny and evidence of cyto-nuclear co-adaptation in Arabidopsis thaliana. Plant J 63:728-738.

Duroc, Y., S. Hiard, N. Vrielynck, S. Ragu and F. Budar (2009). The Ogura sterility-inducing protein forms a large complex without interfering with the oxidative phosphorylation components in rapeseed mitochondria. Plant Mol Biol 70(1-2) :123-37.

Uyttewaal, M., N. Arnal, M. Quadrado, A. Martin-Canadell, N. Vrielynck, S. Hiard, H. Gherbi, A. Bendahmane, F. Budar and H. Mireau (2008b). "Characterization of Raphanus sativus Pentatricopeptide Repeat Proteins Encoded by the Fertility Restorer Locus for Ogura Cytoplasmic Male Sterility." Plant Cell 20(12): 3331-45.

Berthome, R. Thomasset, M. Maene, M. Bourgeois, N. Froger, N. Budar, F. (2008) pur4 mutations are lethal to the male, but not the female gametophyte and affect sporophyte development in Arabidopsis Plant Physiol 147(2) 650-60

Gonzalez-Melendi, P. Uyttewaal, M. Morcillo, C. N. Hernandez Mora, J. R. Fajardo, S. Budar, F. Lucas, M. M. (2008) A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus). J Exp Bot 59(4) 827-38

Uyttewaal M, Mireau H, Rurek M, Hammani K, Arnal N, Quadrado M, Giege P. (2008a) PPR336 is associated with polysomes in plant mitochondria. J Mol Biol 375(3) 626-36.

Giancola, S., Y. Rao, S. Chaillou, S. Hiard, A. Martin-Canadell, G. Pelletier and F. Budar (2007) Cytoplasmic suppression of Ogura cytoplasmic male sterility in European natural populations of Raphanus raphanistrum. Theor Appl Genet, 114:1333-1343.

Arnal N, Alban C, Quadrado M, Grandjean O, Mireau H. (2006) The Arabidopsis Bio2 protein requires mitochondrial targeting for activity. Plant Mol Biol 62 : 471-479.

Duroc, Y, Gaillard, C., Hiard, S., Tinchant, C, Berthomé, R, Pelletier, G and Budar, F (2006) « Nuclear expression of a cytoplasmic male sterility gene modifies mitochondrial morphology in yeast and plant cells. » Plant Science 170 :755-767. (pdf -intranet uniquement)

Reviews

Pelletier, G. and F. Budar (2007) The molecular biology of cytoplasmically inherited male sterility and prospects for its engineering. Curr Opin Biotechnol 18(2): 121-125.

Budar F, Berthomé R. (2007) Cytoplasmic male sterilities and mitochondrial gene mutations in land plants. Logan, D. C. Plant Mitochondria. Blackwell Publishing 31 278-307.

Budar F, Touzet P & Pelletier G "Cytoplasmic male sterility" In Flowering and its manipulation, Annual Plant Reviews, vol 20 (C. Ainsworth Ed) Blackwell Publishing 2006.