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  Morphogenesis, Signaling, Modeling
  Dynamics and Expression of plant Genomes
  Adaptation of Plants to the Environment
  Reproduction and Seeds
  Plant cell wall, function and utilization
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Keywords : abiotic stress, abscisic acid, after-ripening, Arabidopsis thaliana, biosynthesis, carotenoid, compartmentalization, condensed tannins, development, dormancy, flavonoid, germination, hormone, longevity, mucilage, mutant, Oryza sativa, nitric oxide, pectin, post-translational modification, proanthocyanidines, proteomics, secondary metabolism, seed, tegument, transparent testa, transport.

Doctoral school affiliation : ED435 ABIES, ED 567 Sciences du Végétal

Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech-ERL3559 CNRS
INRA - Centre de Versailles-Grignon
Bâtiment 2
Route de Saint-Cyr (RD 10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19

Loïc Rajjou
University Lecturer

François Perreau

Béatrice Godin
Technician AgroParisTech

Gwendal Cueff
Technician AgroParisTech 50%

Boris Collet
Technician Assistant AgroParisTech 50%

Hiromi Suzuki
Post-Doc Agreenskills



Frédéric Chauffour
PhD student


Omaé Pozza
Engineer contractual

Annie Marion-Poll
Senior scientist

Isabelle Debeaujon
Research Scientist

Adeline Berger
Assistant Engineer 50%

Elodie Akary
Assistant Engineer

Christine Sallé
Technician Assistant


Naoto Sano

Isabelle Fabrissin
PhD student



Summary :

Research in the group ‘Germination physiology’ is focused on key factors and processes that regulate seed dormancy, longevity and adaptation to environmental constraints, with the aim of improving seed germination and vigor. A combination of molecular genetics, functional genomics, transcriptomics, proteomics, metabolomics, physiology and histology are being employed, mainly in Arabidopsis but also in rice.
Abscisic acid (ABA) plays a major role in the induction of seed dormancy and the inhibition of germination in response to abiotic stress. To improve our understanding of ABA function, we are identifying and characterizing genes involved in its production and signal transduction in seeds. We are also studying a positive regulator of seed germination and stress tolerance, nitric oxide (NO°). In particular we are examining its ability to modulate protein function by posttranslational modification (S-nitrosylation, nitration…), in relation to the physiological state of seeds. Other protein modifications that impact on seed longevity and survival in adverse conditions, are also being investigated, notably the role of the protein L-isoaspartate O-methyltransferase (PIMT). This enzyme repairs proteins, which have undergone conformational changes due to spontaneous isomerization or deamination of aspartate and asparagine residues.
Germination characteristics are also influenced by seed coat properties and work in our group is aimed at understanding the role of two types of metabolites accumulated in this organ, flavonoids and mucilage polysaccharides. Flavonoids are secondary metabolites that contribute to seed protection against biotic and abiotic stresses and also affect seed agronomic quality (dormancy, germination and longevity). Our current research aims at understanding the mechanisms regulating flavonoid metabolism (biosynthesis, intracellular trafficking and storage) and their integration in Arabidopsis seed development. The polysaccharides accumulated in the outer cell layer of the seed coat are liberated on hydration to form a mucilage around the seed. The synthesis and structure of mucilage is complex, and its physiological role unclear. We are exploiting natural mucilage mutants to identify new genes involved in its production and to gain insight into its function.

Main Results :

Hormonal regulation of germination
PI: Annie Marion-Poll

Abscisic acid is a key regulator of seed development and germination as well as adaptive responses to environmental stresses. Modulation of its endogenous levels by fine-tuning of synthesis and catabolism determines its physiological action, together with its perception and signal transduction. ABA is synthesized from the C40 oxygenated carotenoids. Our group has contributed to unraveling the biosynthesis pathway by the isolation of genes involved in violaxanthin and neoxanthin synthesis, and the functional analysis of the NCED gene family that encodes a 9-cis epoxycarotenoid dioxygenase, responsible for carotenoid cleavage. We are currently investigating the tissue specific regulation of ABA biosynthesis in Arabidopsis seeds, in order to identify regulatory factors.
Current knowledge about the ABA signaling network is still incomplete, especially in seeds, although the recent identification of ABA receptors constitutes a major breakthrough. Using infrared thermography, we have selected suppressors of the ABA-deficient mutant Ataba3. None of these suppressor mutations modify ABA levels and current evidence indicates that they affect ABA signaling. Identification of three of these suppressor loci is underway.

Seed proteomics
PI: Loïc Rajjou

Seed dormancy is released during dry storage (after-ripening) resulting in increased germination ability. Prolonged storage can, however, lead to alterations that affect the potential of seeds to germinate. Team members are currently investigating the molecular basis of the control of seed germination by functional proteomics in two model plants, Arabidopsis thaliana and Oryza sativa. In particular, we are studying the effects of nitrate (NO3) and nitric oxide (NO°) on the molecular control of germination (dormancy, vigor, and longevity). Indeed, in addition to the influence of the ABA/GA hormonal balance, recent research has shown that NO° can break seed dormancy and promote germination in stress conditions. The mode of action of this radical in seeds and its preferential molecular targets remain unknown. A number of recent investigations have highlighted different protein modifications that can be produced by NO°, namely nitrosylation of metal and thiol centers and nitration of tyrosine residues, each leading to different and specific signaling effects. We aim to characterize proteins undergoing NO-mediated post-translational modifications (PTMs) in seeds subjected to different physiological conditions.

Seed mucilage production and function
PI: Helen North

Approaches that exploit the natural variation in Arabidopsis have been developed over recent years at Versailles. In particular, our group has characterized variant loci that are involved in the adaptation of germination to environmental conditions. Analysis of the Versailles collection of accessions identified differences in germination capacity at low temperature in the dark as well as in mucilage production. A recombinant inbred line population, generated from the accessions Bay-0 and Shahdara, was used to identify QTL affecting germination in the cold and dark. These two accessions also differed in their capacity to release seed mucilage on imbibition. We have studied the physicochemical properties of this mucilage and isolated a gene affecting the hygroscopic properties of the pectins that constitute the mucilage. Currently, within the framework of an ANR-blanc project (AMUSE) our group is studying the natural variability of Arabidopsis mucilage composition.

Metabolism and biological roles of seed flavonoids
PI: Isabelle Debeaujon

Arabidopsis seeds accumulate condensed tannins (proanthocyanidins) specifically in the inner integument, and flavonols in all seed compartments. The objectives of our group are to identify and/or characterize the different functions that are required for the biosynthesis, transport and subcellular compartmentation of these flavonoids. We have determined that a laccase encoded by the TRANSPARENT TESTA 10 (TT10) gene catalyzes the browning and oxidative polymerization of flavonoids (Pourcel et al., 2005). In collaboration with the group of Markus Klein (Zürich university), we have also demonstrated that the MATE transporter TT12 is localized at the tonoplast and is able to transport flavonoids (Marinova et al., 2007). Unraveling the function of the TT15 protein in flavonoid biosynthesis is one of our current objectives. A second aspect of our research consists of analyzing the functions of flavonoids in seed physiology and seed tolerance to biotic and abiotic stresses.





INRA Plant Biology and Breeding incentive project ExMu (2013-2015) Exploitation of Arabidopsis seed mucilage for the functional characterisation of genes involved in polysaccharide metabolism (Coordinator Helen North)

ANR-Blanc MITOZEN (2013-2016) Toward the function of plant mitochondrial LEA and sHSP proteins (Coordinator David Macherel, Angers University) - Task 3 Reverse genetics and phenotyping (Leader Annie Marion-Poll)

ANR-BioAdapt CytoPheno (2012-2015) Cyto-nuclear co-adaptation and plant adaptive phenotypes (Coordinator Françoise Budar, IJPB) - Task 4 Seed dormancy and proteomics experiments  (Leader Loïc.Rajjou)

ANR-BioAdapt AutoAdapt (2012-2015) Role of autophagy in plant adaptation to nitrate and sulphate limitation in environment. (Coordinator Céline Masclaux-Daubresse, IJPB) - Task 3 Role of autophagy in seed filing (Leader Loïc Rajjou)

FP7-KBBE Ecoseed (2013-2016) Impacts of Environmental Conditions on Seed Quality (Coordinator Ilse Kranner, Innsbruck University, Austria) - WP3 Molecular changes during imbibition and germination (Coordinator Loïc Rajjou) 


Ogé L, Grappin P, Collet B (2009) S-Adenosine homocysteine quantitation to monitor seed storability and seed vigor. Patent Registration submitted

Marion-Poll A, North H, Lessard P, Redondo E (2006) Identification of proteins useful for improving plant tolerance water deficit (ABA4). Patent n° 06290983.

Grappin P, Ogé L, Bove J (2005) Using L-isoaspartyl methyltransferase as molecular marker of seed ageing. Patent n° 03 13858.

Debeaujon I, Devic M, Caboche M, Lepiniec L (2001) Acide nucléique régulateur permettant l'expression d'un polynucléotide d'intérêt spécifiquement dans l'endothélium d'un grain de plante, et ses applications. Patent n°01-10365.

Selected Publications :

Deruyffelaere C, Purkrtova Z, Bouchez I, Collet B, Cacas J-L, Chardot T, Gallois JL, Andrea S (2018) PUX10 associates with CDC48A and regulates the dislocation of ubiquitinated oleosins from seed lipid droplets. Plant Cell (In press) (doi:10.1105/tpc.18.00275)

Liu B, Honnorat B, Yang H, Arancibia J, Rajjou L, Rousseau A (2018) Non-thermal DBD plasma array on seed germination of different plant species. Journal of Physics D: Applied Physics. (In press)

Sechet J, Marion-Poll A, North HM (2018) Emerging functions for cell wall polysaccharides accumulated during eudicot seed development. Plants (in press) (doi: 10.3390/plants7040081)

Di Berardino J, Marmagne A, Berger A, Yoshimoto K, Cueff G, Chardon F, Masclaux-Daubresse C, Reisdorf-Cren M (2018) Autophagy controls resource allocation and protein storage accumulation in Arabidopsis seeds. J Exp Bot 69:1403-1414

Havé M, Balliau T, Cottyn-Boitte B, Dérond E, Cueff G, Soulay F, Lornac A, Reichman P, Dissmeyer N, Avice JC, Gallois P, Rajjou L, Zivy M, Masclaux-Daubresse C (2018) Increase of proteasome and papain-like cysteine protease activities in autophagy mutants: backup compensatory effect or pro cell-death effect? J Exp Bot, 69:1369-1385

Xia Q, Maharaja P, Cueff G, Rajjou L, Prodhomme D, Gibon Y, Bailly C, Corbineau F, Meimoun P, El-Maarouf-Bouteau H (2018) Integrating proteomics and enzymatic profiling to decipher seed metabolism affected by temperature in seed dormancy and germination. Plant Sci 269: 118-125

Chedraoui S, Abi-Rizk A, El-Beyrouthy M, Chalak L, Ouaini N, Rajjou L (2017) Capparis spinosa L. in a systematic review: a xerophilous species of multi values and promising potentialities for agrosystems under the threat of global warming. Front Plant Sci 8: 1845

Galland M, He D, Lounifi I, Arc E, Clément G, Balzergue S, Huguet S, Cueff G, Godin B, Collet B, Granier F, Morin H, Tran J, Valot B, Rajjou L (2017) An integrated "Multi-Omics" comparison of embryo and endosperm tissue-specific features and their impact on rice seed quality. Front Plant Sci 8:1984

Griffiths JS, North HM (2017) Sticking to cellulose: exploiting Arabidopsis seed coat mucilage to understand cellulose biosynthesis and cell wall polysaccharide interactions. New Phytol 214, 959-966

Leung J (2017) The first broad-spectrum abscisic acid antagonist. Plant Physiol 173, 1939

Dekkers B, He H, Hanson J, Willems L, Jamar D, Cueff G, Rajjou L, Hilhorst H, Bentsink L (2016). The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5  (ABI5) expression and genetically interacts with ABI3during Arabidopsis seed development. Plant J 85, 451-465

Feilke K, Streb P, Cornic G, Perreau F, Kruk J, Krieger-Liszkay A (2016) Effect of Chamydomonas plastid terminal oxidase 1 expressed in tobacco on photosynthetic electron transfer. Plant J 85, 219-228

Fendrych M, Leung J, Friml J (2016) TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. eLife 5

Griffiths JS, Crépeau M-J, Ralet M-C, Seifert GJ, North HM (2016) Dissecting seed mucilage adherence mediated by FEI2 and SOS5. Front Plant Sci 7, 1073

Leung J, Benzihizina N, Mancuso S and Valon C (2016) Revisiting the plant's dilemma. Mol Plant 9, 7-9

López-Igual R, Wilson A, Leverenz RL, Melnicki MRM, Bourcier de Carbon C, Sutter M, Turmo A, Perreau F, Kerfeld CA, Kirilovsky D (2016) Different functions of the paralogs to the N-terminal domain of the Orange Carotenoid Protein in the cyanobacterium Anabaena sp. PCC 71201. Plant Physiol 171, 1852-1866

Melnicki MR, Leverenz RL, Sutter M, López-Igual R, Wilson A, Pawlowski EG, Perreau F, Kirilovsky D, Kerfeld CA (2016) Structure, diversity and evolution of a new family of soluble carotenoid binding proteins in cyanobacteria. Mol Plant 9, 1379-1394

Ralet M-C, Crépeau M-J, Vigoroux J, Tran J,  Berger A, Sallé C, Granier F, Botran L, North HM (2016) Xylans provide the structural driving force for mucilage adhesion to the Arabidopsis seed coat. Plant Physiol 171, 165-178

Sano N, Rajjou L, North HM, Debeaujon I, Marion-Poll A, Seo M (2016) Staying alive: molecular aspects of seed longevity. Plant Cell Physiol 57, 660-674

Sechet J, Frey A, Effroy-Cuzzi D, Berger A, Perreau F, Cueff G, Charif D, Rajjou L, Mouille G, North HM, Marion-Poll A (2016) Xyloglucan metabolism differentially impacts the cell wall characteristics of the endosperm and embryo during Arabidopsis seed germination. Plant Physiol 170, 1367-1380

Seo M, Kanno Y, Frey A, North HM, Marion-Poll A (2016) Dissection of Arabidopsis NCED9 promoter regulatory regions reveals a role for ABA synthesized in embryos in the regulation of GA-dependent seed germination. Plant Sci 246, 91-97

Voiniciuc C, Zimmermann E, Schmidt MH, Gunl M, Fu L, North HM, Usadel B (2016) Extensive natural variation in Arabidopsis seed mucilage structure. Front Plant Sci 7, 803

Bourcier de Carbon C, Thurotte A, Wilson A, Perreau F, Kirilovsky D (2015) Biosynthesis of soluble carotenoid holoproteins in Escherichia coli cells. Scientific Reports 5, 9085

Galland M, Rajjou L (2015) Regulation of mRNA translation controls seed germination and is critical for seedling vigor. Front Plant Sci 6, 284

Leung J, Bazihizina N, Mancuso S, Valon C (2015) Revisiting the plant's dilemma. Mol Plant 10, 1016

Leverenz RL, Sutter M, Wilson A, Gupta S, Thurotte A, Bourcier de Carbon C, Petzold CJ, Ralston C, Perreau F, Kirilovsky D, Kerfeld CA (2015) A 12 Å carotenoid translocation in a photoswitch associated with cyanobacterial photoprotection. Science 348, 1463-1466

Nguyen TP, Cueff G, Hegedus DD, Rajjou L, Bentsink L (2015) A role for seed storage proteins in Arabidopsis seed longevity. J Exp Bot 66, 6399-6341

Poncet V, Scutt C, Tournebize R, Villegente M, Cueff G, Rajjou L, Balliau T, Zivy M, Fogliani B, Job C, de Kochko A, Sarramegna-Burtet V, Job D (2015) The Amborella vacuolar processing enzyme family. Front Plant Sci 21, 618

Sechet J, Roux C, Plessis A, Effroy D, Frey A, Perreau F, Biniek C, Krieger-Liszkay A, Macherel D, North HM, Mireau H, Marion-Poll A (2015) The ABA-deficiency suppressor locus HAS2 encodes the PPR protein LOI1/MEF11 involved in mitochondrial RNA editing. Mol Plant 8, 1-13

Vilela B, Nájar E, Lumbreras V, Leung J, Pagès M (2015) Casein kinase 2 negatively regulates abscisic acid-activated SnRK2s in the core abscisic acid-signaling module. Mol Plant 5, 709-721

Voiniciuc C, Schmidt MH, Berger A, Yang B, Ebert B, Scheller HV, North HM, Usadel B, Guenl M (2015) MUCI10 produces galactoglucomannan that maintains pectin and cellulose architecture in Arabidopsis seed mucilage. Plant Physiol 169, 403-420

David L, Dechorgnat J, Berquin P, Routaboul JM, Debeaujon I, Daniel-Vedele F, Ferrario-Méry S (2014) Proanthocyanidin oxidation of Arabidopsis seeds is altered in mutant of the high-affinity nitrate transporter NRT2.7. J Exp Bot 65, 885-893

Galland M, Boutet-Mercey S, Lounifi I, Godin-Pérennez B, Aubourg-Balzergue S, Grandjean O, Morin H, Perreau F, Debeaujon I, Rajjou L (2014) Compartmentation and dynamics of flavone metabolism in dry and germinated rice seeds. Plant Cell Physiol 55, 1646-1659

Galland M, Huguet R, Arc E, Cueff G, Job D, Rajjou L (2014) Dynamic proteomics emphasizes the importance of selective mRNA translation and protein turnover during Arabidopsis seed germination. Mol Cell Proteomics 13, 252-268

Jallet D, Thurotte A, Leverenz RL, Perreau F, Kerfeld CA, Kirilovsky D (2014) Specificity of the cyanobacterial Orange Carotenoid Protein: Influences of OCP and phycobilisome structures. Plant Physiol 164, 790-804

Jammes F, Leonhardt N, Tran D, Bousserouel H, Véry AA, Renou JP, Vavasseur A, Kwak JM, H Sentenac, F Bouteau, Leung J (2014) Acetylated 1,3 -diaminopropane antagonizes abscisic acid-mediated stomatal closing in Arabidopsis. Plant J 79, 322-333

North HM, Berger A, Saez-Aguayo S, Ralet MC (2014) Understanding polysaccharide production and properties using seed coat mutants: future perspectives for the exploitation of natural variants. Ann Bot 114, 1251-1263

Saez-Aguayo S, Rondeau-Mouro C, Macquet A, Kronholm I, Ralet MC, Berger A, Salle C, Poulain D, Granier F, Botran L, Loudet O, de Meaux J, Marion-Poll A, North HM (2014) Local evolution of seed flotation in Arabidopsis. PLoS Genet 10, e1004221

Sedoud A, López-Igual R, Ur Rehman A, Wilson A, Perreau F, Boulay C, Vass I, Krieger-Liszkay A, Kirilovsky D (2014) The cyanobacterial photoactive orange carotenoid protein is an excellent singlet oxygen quencher. Plant Cell 26, 1781-1791

Albert VA, Barbazuk WB, dePamphilis CW, Der JP, Leebens-Mack J, Ma H, Palmer JD, Rounsley S, Sankoff D, Schuster SC, Soltis DE, Soltis PS, Wessler SR, Wing RA, Albert VA, Ammiraju JS, Barbazuk WB, Chamala S, Chanderbali AS, dePamphilis CW, Der JP, Determann R, Leebens-Mack J, Ma H, Ralph P, Rounsley S, Schuster SC, Soltis DE, Soltis PS, Talag J, Tomsho L, Walts B, Wanke S, Wing RA, Albert VA, Barbazuk WB, Chamala S, Chanderbali AS, Chang TH, Determann R, Lan T, Soltis DE, Soltis PS, Arikit S, Axtell MJ, Ayyampalayam S, Barbazuk WB, Burnette JM 3rd, Chamala S, De Paoli E, dePamphilis CW, Der JP, Estill JC, Farrell NP, Harkess A, Jiao Y, Leebens-Mack J, Liu K, Mei W, Meyers BC, Shahid S, Wafula E, Walts B, Wessler SR, Zhai J, Zhang X, Albert VA, Carretero-Paulet L, dePamphilis CW, Der JP, Jiao Y, Leebens-Mack J, Lyons E, Sankoff D, Tang H, Wafula E, Zheng C, Albert VA, Altman NS, Barbazuk WB, Carretero-Paulet L, dePamphilis CW, Der JP, Estill JC, Jiao Y, Leebens-Mack J, Liu K, Mei W, Wafula E, Altman NS, Arikit S, Axtell MJ, Chamala S, Chanderbali AS, Chen F, Chen JQ, Chiang V, De Paoli E, dePamphilis CW, Der JP, Determann R, Fogliani B, Guo C, Harholt J, Harkess A, Job C, Job D, Kim S, Kong H, Leebens-Mack J, Li G, Li L, Liu J, Ma H, Meyers BC, Park J, Qi X, Rajjou L, Burtet-Sarramegna V, Sederoff R, Shahid S, Soltis DE, Soltis PS, Sun YH, Ulvskov P, Villegente M, Xue JY, Yeh TF, Yu X, Zhai J, Acosta JJ, Albert VA, Barbazuk WB, Bruenn RA, Chamala S, de Kochko A, dePamphilis CW, Der JP, Herrera-Estrella LR, Ibarra-Laclette E, Kirst M, Leebens-Mack J, Pissis SP, Poncet V, Schuster SC, Soltis DE, Soltis PS, Tomsho L (2013) The Amborella genome and the evolution of flowering plants. Science. 342:1241089

Arc E, Galland M, Godin B, Cueff G, Rajjou L (2013) Nitric oxide implication in the control of seed dormancy and germination. Front Plant Sci 4, 346

Vilela B, Moreno-Cortés A, Rabissi A, Leung J, Pagès M, Lumbreras V (2013) The maize OST1 kinase homolog phosphorylates and regulates the maize SNAC1-type transcription factor. PLoS One 8:e58105

Arc E, Sechet J, Corbineau F, Rajjou L, Marion-Poll A (2013) ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Front Plant Sci 4, 63

Lounifi I, Arc E, Molassiotis A, Job D, Rajjou L, Tanou G (2013) Interplay between protein carbonylation and nitrosylation in plants. Proteomics 13, 568-578

Saez-Aguayo S, Ralet MC, Berger A, Botran L, Marion-Poll A, North HM (2013) PECTIN METHYLESTERASE INHIBITOR6 promotes Arabidopsis mucilage release by limiting methylesterification of homogalacturonan in seed coat epidermal cells. Plant Cell 25, 308-323

Arc E, Chibani K, Grappin P, Jullien M, Godin B, Cueff G, Valot B, Balliau T, Job D, Rajjou L (2012) Cold stratification and exogenous nitrates entail similar functional proteome adjustments during Arabidopsis seed dormancy release. J Proteome Res 11, 5418-5432

Berriri S, Garcia AV, Frei dit Frey N, Rozhon W, Pateryron S, Leonhardt N, Montillet J-L, Leung J, Hirt H, Colombet J (2012) Constitutively active mitogen-activated protein kinase versions reveal functions of Arabidopsis MPK4 in pathogen defence signaling. Plant Cell 24, 4281-4293

Leung J (2012) Controlling hormone action by subversion and deception. Science 335, 46-47

Arc E, Chibani K, Grappin P, Jullien M, Godin B, Cueff G, Valot B, Balliau T, Job D, Rajjou L (2012) Cold stratification and exogenous nitrates entail similar functional proteome adjustments during Arabidopsis seed dormancy release. J Proteome Res 11, 5418-5432 

Berthet S, Thevenin J, Baratiny D, Demont-Caulet N, Debeaujon I, Bidzinski P, Leple JC, Huis R, Hawkins S, Gomez LD, Lapierre C, Jouanin, L (2012) Role of plant laccases in lignin polymerization. In Lignins, biosynthesis, biodegradation and bioengineering. In Advances in Botanical Research 61, Jouanin L and Lapierre C eds (Elsevier Ltd), pp 145-172

Denancé N, Ranocha P, Oria N, Barlet X, Develey-Rivière MP, Yadeta K, Hoffmann L, Perreau F, Gilles, C, Maia-Grondard, A, Berg G, Savelli B, Fournier S, Aubert Y, Pelletier, S, Thomma, B, Molina A, Jouanin L, Marco Y, Goffner D (2012) Arabidopsis wat1 (walls are thin1)-mediated resistance to the bacterial vascular pathogen, Ralstonia solanacearum, is accompanied by cross-regulation of salicylic acid and indole metabolism. Plant J 73, 225-239

Frey A, Effroy D, Lefebvre V, Seo M, Perreau F, Berger A, Sechet J, To A, North HM, Marion-Poll A (2012) Epoxycarotenoid cleavage by NCED5 fine-tunes ABA accumulation and affects seed dormancy and drought tolerance with other NCED family members. Plant J 70, 501-512 

Galland M, Job D, Rajjou L (2012) The seed proteome web portal. Front Plant Sci 3, 98

Galland M, Lounifi I, Cueff G, Baldy A, Morin H, Job D, Rajjou L (2012) A role for "omics" technologies in exploration of the seed nutritional quality. In SEED DEVELOPMENT: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. Eds Agrawal GK & Rakwal R, Springer, pp 477-501 

Pochon S, Terrasson E, Guillemette T, Iacomi-Vasilescu B, Georgeault S, Juchaux M, Berruyer R, Debeaujon I, Simoneau P, Campion C (2012) The Arabidopsis thaliana - Alternaria brassicicola pathosystem: a model interaction for investigating seed transmission of necrotrophic fungi. Plant Methods 8, 16

Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C, Job D (2012) Seed Germination and Vigor. Annual Review of Plant Biology 63, 507-53

Joshi-Saha A, Valon C, Leung J (2011) Abscisic acid signal off the STARTing block. Mol Plant 4, 562-580

Joshi-Saha A, Valon C, Leung J (2011) A brand new START: Abscisic acid perception and transduction in the guard cell. Science Signaling 4, re4

Lechner E, Leonhardt N, Eisler H, P armentier Y, Alioua M, Jacquet H, Leung J, Genschik P (2011) MATH/BTB CRL3 receptors target the homeodomain-leucine zipper AtHB6 to modulate abscisic acid signaling. Dev Cell 21, 1116-1128

Lefebvre V, Fortabat MN, Ducamp A, North HM, Maia-Grondard A, Trouverie J, Boursiac Y, Mouille G, Durand-Tardif M (2011) ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport. PLoS One 6, e16645

Rajjou L, Belghazi M, Catusse J, Ogé L, Arc E, Godin B, Chibani K, Ali-Rachidi S, Collet B, Grappin P, Jullien M, Gallardo K, Job C, Job D (2011) Proteomics and posttranslational proteomics of seed dormancy and germination. Methods Mol Biol 773, 215-36

Sullivan JS, Ralet MC, Berger A, Diatloff E, Bishoff V, Gonneau M, Marion-Poll A, North HM (2011) CESA5 is required for the synthesis of cellulose with a role in structuring the adherent mucilage of Arabidopsis seeds. Plant Physiol 156, 1725-1739 1

Wilson A, Punginelli C, Couturier M, Perreau F, Kirilovsky D (2011) Essential role of two tyrosines and two tryptophans on the photoprotection activity of the Orange Carotenoid Protein. Biochim Biophys Acta 1807, 293-30

Arc E, Galland M, Cueff G, Godin B, Lounifi I, Job D, Rajjou L (2011) Reboot the system thanks to protein post-translational modifications and proteome diversity: How quiescent seeds restart their metabolism to prepare seedling establishment. Proteomics 11, 1606-1618

Arc E, Ogé L, Grappin P, Rajjou L (2011) Plant Seed: A Relevant Model to Study Aging Processes. in "The Field of Biological Aging: Past, Present and Future", Research Signpost, Olgun A Ed, pp 87-102

Grappin P, Collet B, Yang H, Jallet D, Oge L, Zubarev R (2011). New proteomic developments to analyze protein isomerization and their biological significance in plants. J Proteomics 74, 1475-1482

Lefebvre V, Fortabat MN, Ducamp A, North HM, Maia-Grondard A, Trouverie J, Boursiac Y, Mouille G, Durand-Tardif M (2011) ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport. PLoS One 6, e16645

Ogé L, Broyart C, Collet B, Godin B, Jallet D, Bourdais G, Job D, Grappin P (2011) Protein damage and repair controlling seed vigor and longevity. Methods Mol Biol 773, 369-84

Plessis A, Cournol R, Effroy E, Silva Pérez V, Botran L, Kraepiel Y, Frey A, Sotta B, Cornic G, Leung J, Giraudat J, Marion-Poll A, North HM (2011) New ABA-hypersensitive Arabidopsis mutants are affected in loci mediating responses to water deficit and Dickeya dadantii infection. PLoS One 6, e20243

Rajjou L, Belghazi M, Catusse J, Ogé L, Arc E, Godin B, Chibani K, Ali-Rachidi S, Collet B, Grappin P, Jullien M, Gallardo K, Job C, Job D (2011) Proteomics and posttranslational proteomics of seed dormancy and germination. Methods Mol Biol 773, 215-36

Sullivan JS, Ralet MC, Berger A, Diatloff E, Bishoff V, Gonneau M, Marion-Poll A, North HM (2011) CESA5 is required for the synthesis of cellulose with a role in structuring the adherent mucilage of Arabidopsis seeds. Plant Physiol 156, 1725-1739 

Wilson A, Punginelli C, Couturier M, Perreau F, Kirilovsky D (2011) Essential role of two tyrosines and two tryptophans on the photoprotection activity of the Orange Carotenoid Protein. Biochim Biophys Acta 1807, 293-301


Dubreucq B, Baud S, Debeaujon I, Dubos C, Marion-Poll A, Miquel M, North H, Rochat C, Routaboul JM, Lepiniec L (2010) Seed development. Chapter 17, In: Plant Developmental Biology – Biotechnological Perspectives: Volume 1, E.C. Pua and M.R. Davey (eds.), DOI 10.1007/978-3-642-02301-9_17, Springer-Verlag Berlin Heidelberg, Part 3, 341-359

North H, Baud S, Debeaujon I, Dubos C, Dubreucq B, Grappin P, Jullien M, Lepiniec L, Marion-Poll A, Miquel M, Rajjou L, Routaboul JM, Caboche M (2010) Arabidopsis seed secrets unravelled after a decade of genetic and omics-driven research. Plant J 61, 971-981

Ogé L, Broyart C, Collet B, Jallet D, Bourdais G, Job D, Grappin P (2010) Protein damage and repair controlling seed vigor and longevity. In Seed Dormancy (Ed A Kermode) Methods and Molecular Biol Humana Press Inc (USA), in press

Wilson A, Kinney JN, Zwart PH, Punginelli C, D'Haene S, Perreau F, Klein MG, Kirilovsky D, Kerfeld CA (2010) Structural determinants underlying photoprotection in the photoactive Orange Carotenoid Protein of cyanobacteria. J Biol Chem 285, 18364-375

Nesi N, Lucas M-O, Auger B, Baron C, Lécureuil A, Guerche P, Kronenberger J, Lepiniec L, Debeaujon I, Renard M (2009) The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat. Plant Cell Rep 28, 601-617

Punginelli C, Wilson A, Routaboul JM, Kirilovsky D (2009) Influence of zeaxanthin and echinenone binding on the activity of the Orange Carotenoid Protein. Biochim Biophys Acta 1787, 280-288

Sugliani M, Rajjou L, Clerkx EJM, Koornneef M, Soppe WJJ (2009) Natural modifiers of seed longevity in the Arabidopsis mutants abi3-5 and lec1-3. New Phytol 184, 898-908

Tanou G, Job C, Rajjou L, Arc E, Belghazi M, Diamantidis G, Molassiotis A, Job D (2009) Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity. Plant J 60, 795-804

Barrero JM, Rodríguez PL, Quesada V, Alabadí D, Blázquez MA, Boutin JP, Marion-Poll A, Ponce MR, Micol JL (2008) The ABA1 gene and carotenoid biosynthesis are required for late skotomorphogenic growth in Arabidopsis thaliana. Plant Cell Env 31, 227–234

Catusse J, Rajjou L, Job C, Job D (2008) Seed: Germination to development. In Plant Proteomics: Technologies, Strategies, and Applications. Eds. John Wiley & Sons, Inc, pp.152-166

Dubos C, Le Gourrierec J, Baudry A, Huep G, Lanet E, Debeaujon I, Routaboul J-M, Alboresi A, Weisshaar B, Lepiniec L (2008) MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana. Plant J 55, 940-953

Grappin P (2008) Seed aging and survival mechanisms. J Biol Sci 202, 231-239

Grappin P, Bourdais G, Collet B, Godin B, Job D, Ogé L, Jullien M, Rajjou L (2008) Seed aging and survival mechanisms. J Soc Biol 202, 231-239

Grappin P (2008) Seed conservation and research. Improving molecular markers to optimise long-term germplasm conservation. ENSCoNews, 4, 9-11

Holdsworth MJ, Finch-Savage W, Grappin P, Job D (2008) Post-genomics dissection of seed dormancy and germination. Trends Plant Sci 13, 7-13

Meng P, Macquet A, Loudet O, Marion-Poll A, North H (2008) Analysis of natural allelic variation controlling Arabidopsis thaliana seed germinability in response to cold and dark: identification of three major quantitative trait loci. Mol Plant 1, 145-154

Ogé L, Bourdais G, Bove J, Collet B, Godin B, Granier F, Boutin JP, Job D, Jullien M, Grappin P (2008) Arabidopsis protein repair L-isoaspartyl methyltransferase1 is involved both in seed survival during storage and in germination vigor. Plant Cell 20, 3022-3037

Rajjou L, Debeaujon I (2008) Seed longevity: survival and maintenance of high germination ability of dry seeds. C R Biol 331, 796-805

Rajjou L, Lovigny Y, Groot SPC, Belghazi M, Job C, Job D (2008) Proteome-wide characterization of seed aging in Arabidopsis. A comparison between artificial and natural aging protocols. Plant Physiol 148, 620-64

Wilson A, Punginelli C, Gall A, Bonetti C, Alexandre M, Routaboul JM, Kerfeld CA, van Grondelle R, Robert B, Kennis JTM, Kirilovsky D (2008) A photoactive carotenoid protein acting as light intensity sensor. Proc Nat Acad Sci USA 105, 12075-12080

Dall'Osto L, Cazzaniga S, North H, Marion-Poll A, Bassi R (2007) The Arabidopsis aba4-1 mutant reveals a specific function for neoxanthin in protection against photooxidative stress. Plant Cell 19, 1048-1064

Debeaujon I, Lepiniec L, Pourcel L, Routaboul J-M (2007) Seed coat development and dormancy. Chapter 2 , In : Seed Development, Dormancy and Germination ; Ed by K. Bradford & H. Nonogaki ; Blackwell 2007, 25-43

Macquet A, Ralet MC, Kronenberger J, Marion-Poll A, North H (2007) In situ chemical and macromolecular study of the composition of Arabidopsis thaliana seed coat mucilage. Plant Cell Physiol 48, 984-999

Macquet A, Ralet MC, Loudet O, Kronenberger J, Mouille G, Marion-Poll A, North H (2007) A naturally occurring mutation in an Arabidopsis accession affects a ß-galactosidase that increases the hydrophilic potential of rhamnogalacturonan I in seed mucilage. Plant Cell 19, 3990-4006

Marinova K, Pourcel L, Weder B, Schwarz M, Barron D, Routaboul J-M, Debeaujon I, Klein M (2007) The Arabidopsis MATE transporter TT12 acts as a vacuolar flavonoid/H+-antiporter active in proanthocyanidin-accumulating cells of the seed coat. Plant Cell 19, 2023-2038

North H, De Almeida A, Boutin JP, Frey A, To A, Botran L, Sotta B, Marion-Poll A (2007) The Arabidopsis ABA-deficient mutant aba4 demonstrates that the major route for stress-induced ABA accumulation is via neoxanthin isomers. Plant J 50, 810-824

Pourcel L, Routaboul J-M, Cheynier V, Lepiniec L, Debeaujon I (2007) Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant Sci 12, 29-36

Rajjou L, Miché L, Huguet R, Job C, Job D (2007) Proteome and transcriptome profiling to understand seed germination and identify intrinsic markers determining seed quality, germination efficiency and early seedling vigor. In Seeds: Biology, Development and Ecology. Eds. Navie S, Adkins S and Ashmore S, CAB International Publishing, Cambridge, USA, pp. 149-158

Rajjou L, Lovigny Y, Job C, Belghazi M, Groot S, Job D (2007) Seed quality and germination. In Seeds: Biology, Development and Ecology. Eds. Navie S, Adkins S and Ashmore S, CAB International Publishing, Cambridge, USA, pp. 324-332

Frey A, Boutin JP, Sotta B, Mercier R, Marion-Poll A (2006) Regulation of carotenoid and ABA accumulation during the development and germination of Nicotiana plumbaginifolia seeds. Planta 224, 622-632

Chibani K, Ali-Rachedi S, Job C, Job D, Jullien M, Grappin P (2006) Proteomic analysis of seed dormancy. Plant Physiol 142, 1493-1510

De Diego JG, Rodríguez FD, Rodríguez Lorenzo JL, Grappin P, Cervantes E (2006) cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences. J Plant Physiol 163, 452-462

Lefebvre V, North H, Frey A, Sotta A, Seo M, Okamoto M, Nambara E, Marion-Poll A (2006) Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesised in the endosperm is involved in the induction of seed dormancy. Plant J 45, 309-319

Lepiniec L, Debeaujon I, Routaboul J-M, Baudry A, Pourcel L, Nesi N, Caboche M (2006) Genetics and Biochemistry of Seed Flavonoids. Annu Rev Plant Biol 57, 405-430

Marion-Poll A, Leung J (2006) Abscisic acid synthesis, metabolism and signal transduction. In Plant Hormone signaling, Annual Plant Reviews, P Hedden & SG Thomas eds, Blackwell Publishing, Oxford, UK, pp 1-35

Rajjou L, Belghazi M, Huguet R, Robin C, Moreau A, Job C, Job D (2006) Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. Plant Physiol 141, 910-923

Rajjou L, Gallardo K, Job C, Job D (2006) Proteome analysis for the study of developmental processes in plants. In Plant Proteomics. Annual Plant Reviews 28. Ed. Finnie C, Pub. Blackwell Publishing, Oxford, pp. 151-184

Routaboul J-M, Kerhoas L, Debeaujon I, Pourcel L, Caboche M, Einhorn J, Lepiniec L (2006) Flavonoid diversity and biosynthesis in seed of Arabidopsis thaliana. Planta 224, 96-107

Seo S, Hanada A, Kuwahara A, Endo A, Okamoto M, Yamauchi Y, North H, Marion-Poll A, Sun TP, Koshiba T, Kamiya Y, Yamaguchi S, Nambara E (2006) Regulation of hormone metabolism in Arabidopsis seeds: Phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism. Plant J 48, 354-366


Bove J, Lucas P, Godin B, Ogé L, Jullien M, Grappin P (2005) Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia. Plant Mol Biol 57, 593-612

Job C, Rajjou L, Lovigny Y, Belghazi M, Job D (2005) Patterns of protein oxidation in Arabidopsis seeds and during germination. Plant Physiol 138, 790-802
Nambara E, Marion-Poll A (2005) ABA biosynthesis and catabolism. Annu Rev Plant Biol 56, 165-185

North HN, Frey A, Boutin JP, Sotta B, Marion-Poll A (2005) Analysis of xanthophyll cycle gene expression during the adaptation of Arabidopsis to excess light and drought stress: Changes in RNA steady-state levels do not contribute to short-term responses. Plant Sci 169, 115-164

Pourcel L, Routaboul J-M, Kerhoas L, Caboche M, Lepiniec L, Debeaujon I (2005) TRANSPARENT TESTA 10 encodes a laccase-like enzyme involved in oxidative polymerization of flavonoids in Arabidopsis seed coat. Plant Cell 17, 2966-2980

Ali-Rachedi A, Bouinot D, Wagner MH, Bonnet M, Sotta B, Grappin P, Jullien M (2004) Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta 219, 479-488 

Frey A, Godin B, Bonnet M, Sotta B, Marion-Poll A (2004) Maternal synthesis of abscisic acid controls seed development and yield in Nicotiana plumbaginifolia. Planta 218, 958-964

Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job C, Job D (2004) The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiol 134, 1598-1613

Touraine B, Boutin JP, Marion-Poll A, Briat JF, Peltier G, Lobreaux S (2004) Nfu2 : a scaffold protein involved in 4Fe-4S and ferredoxin iron-sulfur cluster assembly in Arabidopsis chloroplasts. Plant J 40, 101-111

Debeaujon I, Nesi N, Perez P, Devic M, Grandjean O, Caboche M, Lepiniec L (2003) Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development. Plant Cell 15, 2514-2531

Nambara E, Marion-Poll A (2003) ABA action and interactions in seeds. Trends Plant Sci 8, 195-244

Chandler PM, Marion-Poll A, Ellis M, Gubler F (2002) Mutants at the Slender1 locus of ‘Himalaya’ barley: molecular and physiological characterization. Plant Physiol 129, 181-190

Merlot S, Mustilli AC, Genty B, North H, Lefebvre V, Sotta B, Vavasseur A, Giraudat J (2002) Use of infrared thermal imaging to isolate Arabidopsis mutants defective in stomatal regulation. Plant J 30, 601-609

Nesi N, Debeaujon I, Jond C, Stewart AJ, Jenkins GI, Caboche M, Lepiniec L (2002) Arabidopsis TRANSPARENT TESTA16 is a MADS-box gene required for proper development and pigmentation of the seed coat. Plant Cell 14, 2463-2479

Audran C, Liotenberg S, Gonneau M, North H, Frey A, Tap-Waksman K, Vartanian N, Marion-Poll A (2001) Localisation and expression of zeaxanthin epoxidase mRNA in Arabidopsis in response to drought stress and during seed development. Aust J Plant Physiol 28, 1161-1173

Borel C, Audran C, Frey A, Marion-Poll A, Tardieu F, Simonneau T (2001) Nicotiana plumbaginifolia zeaxanthin epoxidase transgenic lines have unaltered baseline ABA accumulations in roots and xylem sap, but contrasting sensitivities of ABA accumulation to water deficit. J Exp Bot 52, 427-434

Borel C, Frey A, Marion-Poll A, Tardieu F, Simonneau T (2001) Does engineering ABA biosynthesis in Nicotiana plumbaginifolia modify stomatal response to drought ? Plant Cell Environment 24, 477-489

Bove J, Jullien M, Grappin P (2001) Functional genomics in the study of seed germination. Genome Biol 3, 1002-1005

Debeaujon I, Peeters AJM, Léon-Kloosterziel KM, Koornneef M (2001) The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed Coat endothelium. Plant Cell 13, 853-872

Nesi N, Jond N, Debeaujon I, Caboche M, Lepiniec L (2001) The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seeds. Plant Cell 13, 2099-2114


Debeaujon I, Léon-Kloosterziel KM, Koornneef M (2000) Influence of the testa on seed dormancy, germination and longevity in Arabidopsis. Plant Physiol 122, 403-413

Debeaujon I, Koornneef M (2000) Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid. Plant Physiol 122, 415-424

Grappin P, Bouinot D, Sotta B, Miginiac E, M Jullien (2000) Control of seed dormancy in Nicotiana plumbaginifolia: A post imbibition abscisic acid synthesis imposes dormancy maintenance. Planta 210, 279-285

Koornneef M, Alonso-Blanco C, Bentsink L, Blankestijn-de Vries H, Debeaujon I, Hanhart CJ, Léon-Kloosterziel KM, Peeters AJM, Raz V (2000) The genetics of seed dormancy in Arabidopsis thaliana. In: CAB International. Dormancy in Plants (eds J.-D. Viémont & J. Crabbé), 365-373

Nesi N, Debeaujon I, Jond C, Pelletier G, Caboche M, Lepiniec L (2000) The TT8 gene encodes a basic helix-loop-helix domain protein required for the expression of DFR and BAN genes in Arabidopsis siliques. Plant Cell 12, 1863-1878

Seo M, Peeters AJM, Koiwai H, Oritani T, Marion-Poll A, Zeevaart JAD, Koornneef M, Kamiya Y, Koshiba T (2000) The Arabidopsis aldehyde oxidase 3 (AAO3) gene product catalyzes the final step in abscisic acid biosynthesis in leaves. Proc Natl Acad Sci USA 97, 12908-12913

Devic M, Guilleminot J, Debeaujon I, Bechtold N, Bensaude E, Koornneef M, Pelletier G, Delseny, M (1999) The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat development. Plant J 19, 387-398

Frey A, Audran C, Marin E, Sotta B, Marion-Poll A (1999) Engineering seed dormancy by the modification of zeaxanthin epoxidase gene expression. Plant Mol Biol 39, 1267-1274

Liotenberg S, North H, Marion-Poll A (1999) Molecular biology and regulation of ABA biosynthesis in plants. Plant Physiol Biochem 37, 341-350

Audran C, Borel C, Frey A, Sotta B, Meyer C, Simmoneau T, Marion-Poll A (1998) Expression studies of the zeaxanthin epoxidase gene in Nicotiana plumbaginifolia. Plant Physiol 118, 1021-1028

Sekimoto H, Seo M, Kawakami N, Komano T, Desloire S, Liotenberg S, Marion-Poll A, Caboche M, Kamiya Y, Koshiba T (1998) Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana. Plant Cell Physiol 39, 433-442

Marin E, Marion-Poll A (1997) Tomato flacca mutant is impaired in ABA aldehyde oxidase and xanthine dehydrogenase activities. Plant Physiol Biochem 35, 369-372

Marion-Poll (1997) ABA and seed development. Trends Plant Sci 2, 447-448

Bouvier F, d’Harlingue A, Hugueney P, Marin E, Marion-Poll A, Camara B (1996) Xanthophyll biosynthesis - Cloning, expression, functional reconstitution, and regulation of beta-cyclohexenyl carotenoid epoxidase from pepper (Capsicum annuum). J Biol Chem 271, 28861

Marin E, Nussaume L, Quesada A, Gonneau M, Sotta B, Hugueney P, Frey A, Marion-Poll A (1996) Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to ABA locus of Arabidopsis thaliana. EMBO J 15, 2331-2342




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