Our team elucidate the role of 5 out of the 7 NRT2 members in Arabidopsis:
- In collaboration with the team of Alain Gojon in Montpellier, we showed that NRT2.1 is a plasma-membrane protein, being the main actor of high-affinity nitrate uptake by roots [2, 3].
- The NRT2.7 is expressed on tonoplast and is involved in nitrate storage in seed, playing a role in seed dormancy .
- More recently, in collaboration with our colleagues from Riken Institute, we demonstrated  that NRT2.4 is a very-high affinity nitrate transporter, located n the plasma membrane, which expression is induced during long term nitrogen starvation.
- In collaboration with the team of Mathilde Fagard (IJPB, NPI), we obtained a surprising result from the functional analysis of NRT2.6 protein . It seems to be involved ROS accumulation, making the link between plant responses to biotic and abiotic stresses.
Lezhneva L, Kiba T, Feria-Bourrellier A-B, Lafouge F, Boutet-Mercey S, Zoufan P, Sakakibara H, Daniel-Vedele F, Krapp A (2014) The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen-starved plants. The Plant Journal 80, 230-24 (Pubmed)
David LC , Dechorgnat J, Berquin P, Routaboul JM, Debeaujon I, Daniel-Vedele F and Ferrario-Méry S. (2014). Proanthocyanidin oxidation of Arabidopsis seeds is altered inmutant of the high-affinity nitrate transporter NRT2.7. Journal of Experimental Botany 65: 885–893.
Krapp A, David LC, Chardin C, Girin T, Marmagne A, Leprince AS, Chaillou S, Ferrario-Méry S, Meyer C and Daniel-Vedele F (2014). Nitrate transport and signalling in Arabidopsis. Journal of Experimental Botany, 65: 789–798.
DECHORGNAT J, NGUYEN CT, ARMENGAUD P, JOSSIER M, DIATLOFF E, FILLEUR S, DANIEL-VEDELE F (2011) From the soil to the seeds : the long journey of nitrate in plants. J Exp Bot 62: 1349-1359
FILLEUR S., DORBE M.F., CEREZO M., ORSEL M., GRANIER F., GOJON A., DANIEL-VEDELE F. (2001) An arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate uptake FEBS Letters 489: 220-224
Cerezo M., Tillard P., Filleur S., Munos S., Daniel-Vedele F. and Gojon A. (2001) Major alterations of the regulation of root NO3- uptake are associated with the mutation of NRT2.1 and NRT2.2 genes in Arabidopsis. Plant Physiology127 : 262-71.
Chopin F, Orsel M, Dorbe MF, Chardon F, Truong HN, Miller T, Krapp A, Daniel-Vedele F. (2007) The arabidopsis ATNRT2.7 nitrate transporter gene controls nitrate content in seeds. The Plant Cell 19: 1590-1602.
KIBA T, FERIA-BOURRELIER AB, LAFOUGE F, LEZHNEVA L, BOUTET-MERCEY S, ORSEL M, BREHAUT V, MILLER T, DANIEL-VEDELE F, SAKAKIBARA H, KRAPP A. (2012) The Arabidopsis nitrate transporter NRT2.4 plays a double role in roots ans shoots of nitrogen-starved plants. Plant Cell 24: 245-258.
DECHORGNAT J, PATRIT O, KRAPP A, FAGARD M, DANIEL-VEDELE F. (2012) Characterization of the NRT2.6 gene in Arabidopsis thaliana : a link with plant response to biotic and abiotic stress Plos One 7:e42491.