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High Affinity Nitrate Transporter Family : NRT2

The NRT2 family contains several members in higher plants. In Arabidopsis 7 NRT2 genes are characterized by different expression patterns, depending on the organ and the environmental conditions [1]. In combination with NRT1 and CLC proteins families, they ensure the nitrate uptake from the soil and nitrate distribution in the whole plant.

 

From Dechorgnat et al (2011)
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Main Results

Our team elucidate the role of 5 out of the 7 NRT2 members in Arabidopsis:

- In collaboration with the team of Alain Gojon in Montpellier, we showed that NRT2.1 is a plasma-membrane protein, being the main actor of high-affinity nitrate uptake by roots  [2, 3].

- The NRT2.7 is expressed on tonoplast and is involved in nitrate storage in seed, playing a role in seed dormancy [4].

- More recently, in collaboration with our colleagues from Riken Institute, we demonstrated [5] that NRT2.4 is a very-high affinity nitrate transporter, located n the plasma membrane, which expression is induced during long term nitrogen starvation.

ABCD

 


- In collaboration with the team of Mathilde Fagard (IJPB, NPI), we obtained a surprising result from the functional analysis of NRT2.6 protein [6]. It seems to be involved ROS accumulation, making the link between plant responses to biotic and abiotic stresses.
ABCD2

 

 

Publications

David LC , Dechorgnat J,  Berquin P, Routaboul JM, Debeaujon I, Daniel-Vedele F and Ferrario-Méry S. (2014). Proanthocyanidin oxidation of Arabidopsis seeds is altered inmutant of the high-affinity nitrate transporter NRT2.7. Journal of Experimental Botany 65: 885–893.

Krapp A, David LC, Chardin C, Girin T, Marmagne A, Leprince AS, Chaillou S, Ferrario-Méry S,  Meyer C and Daniel-Vedele F (2014). Nitrate transport and signalling in Arabidopsis. Journal of Experimental Botany, 65: 789–798.

DECHORGNAT J, NGUYEN CT, ARMENGAUD P, JOSSIER M, DIATLOFF E, FILLEUR S, DANIEL-VEDELE F (2011) From the soil to the seeds : the long journey of nitrate in plants. J Exp Bot 62: 1349-1359

FILLEUR S., DORBE M.F., CEREZO M., ORSEL M., GRANIER F., GOJON A., DANIEL-VEDELE F. (2001) An arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate uptake FEBS Letters 489: 220-224

Cerezo M., Tillard P., Filleur S., Munos S., Daniel-Vedele F. and Gojon A. (2001) Major alterations of the regulation of root NO3- uptake are associated with the mutation of NRT2.1 and NRT2.2 genes in Arabidopsis. Plant Physiology127 : 262-71.

Chopin F, Orsel M, Dorbe MF, Chardon F, Truong HN, Miller T, Krapp A, Daniel-Vedele F. (2007) The arabidopsis ATNRT2.7 nitrate transporter gene controls nitrate content in seeds. The Plant Cell 19: 1590-1602.

KIBA T, FERIA-BOURRELIER AB, LAFOUGE F, LEZHNEVA L, BOUTET-MERCEY S, ORSEL M, BREHAUT V, MILLER T, DANIEL-VEDELE F, SAKAKIBARA H, KRAPP A. (2012) The Arabidopsis nitrate transporter NRT2.4 plays a double role in roots ans shoots of nitrogen-starved plants. Plant Cell 24: 245-258.

DECHORGNAT J, PATRIT O, KRAPP A, FAGARD M, DANIEL-VEDELE F. (2012) Characterization of the NRT2.6 gene in Arabidopsis thaliana : a link with plant response to biotic and abiotic stress Plos One 7:e42491.

 

 

 

 
 

 

 

 

 
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