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Keywords : Nitrogen, nitrate, senescence, glutamine synthetase, asparagine synthetase, nitrogen recycling, remobilisation, 15N labelling, signalling, regulating gene expression, transcriptome

Doctoral school affiliation : ED 567 Sciences du Végéta

Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 3
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19

Group leader
Céline Masclaux-Daubresse
Senior Scientist

Fabien Chardon
Research Scientist

Fabienne Soulay



Qinwu Chen
PhD Student

Michèle Reisdorf-Cren
Associate Professor UVSQ

Anne Marmagne

Laurence Bill
Assistant Engineer 50%

Jie Luo

Summary :

The main role of leaf senescence is for nutrient recycling and remobilisation. Leaf senescence has long been investigated and signals able to delay or to increase leaf senescence are numerous. Leaf senescence is also controlled by endogenous signals that depend on genotypes, and differences or delay in the control of autophagy could be one of them.
During vegetative stage, plants store carbon and nitrogen nutrients in their leaves such as starch and proteins. Rubisco accounts for 50% of the total leaf soluble protein content. Together with other photosynthesis-related proteins, Rubisco is then a major storage protein in the leaves. The progressive degradation of chloroplasts during leaf senescence accompanies major modifications in carbon and nitrogen assimilatory pathways, and also the release of nutrients. Numerous senescence-induced cytosolic glutamine synthetase (GS1), asparagine synthetase (AS) and proteases have been isolated. In our group we are studying the carbon and nitrogen metabolism, the physiology and the N-fluxes of mutants affected in GS1 and AS genes.
Autophagy process that involves the formation, expansion and enclosing of double membrane vacuoles named autophagosome around cytoplasmic components for degradation, is enhanced during leaf senescence and in response to several stresses. Recently we have shown that autophagy mutants are impaired in nitrogen remobilisation to the seeds. We are now investigating the role and specificity of autophagy in the degradation and remobilisation of nutrient sources in response to senescence and stresses.



Main Results :

Selected Publications :

Moison M, Marmagne A, Dinant S, Soulay F, Azzopardi M, Lothier J, Citerne S, Morina H, Legay N, Chardon F, Jean- Avice JC, Reisdorf-Cren M & Masclaux-Daubresse C.
(2018). Three cytosolic glutamine synthetase isoforms located in different order veins work together for N remobilization and seed filling in Arabidopsis. Journal of Experimental Botany, in press

Havé M, Balliau T, Cottyn-Boitte B, Derond E, Cueff G, Soulay F, Lornac A, Reichman P, Dissmeyer N, Avice J-C, Gallois P, Rajjou L, Zivy M, & Masclaux-Daubresse C. (2018). Increase of proteasome and papain-like cysteine protease activities in autophagy mutants: backup compensatory effect or pro cell-death effect? Journal of Experimental Botany, 69: 1369-1385,

Havé M, Balliau T, Cottyn-Boitte B, Derond E, Cueff G, Soulay F, Lornac A, Reichman P, Dissmeyer N, Avice J-C, Gallois P, Rajjou L, Zivy M, & Masclaux-Daubresse C. (2018). Increase of proteasome and papain-like cysteine protease activities in autophagy mutants: backup compensatory effect or pro cell-death effect? Journal of Experimental Botany, 69: 1369-1385

Di Berardino J, Marmagne A, Berger A, Yoshimoto K, Cueff G, Chardon F, Masclaux-Daubresse C, & Reisdorf-Cren M. (2018). Autophagy Controls Resource Allocations and Protein Storage Accumulation in Arabidopsis Seeds. Journal of Experimental Botany, 69: 1403-1414

Clément G, Moison M, Soulay F, Reisdorf-Cren M, & Masclaux-Daubresse C. (2018). Metabolomics of laminae and midvein during leaf senescence and source-sink metabolite management in Brassica napus L. leaves. Journal of Experimental Botany, 69:, 891-903.

Gaufichon L, Marmagne A, Belcram K, Yoneyama T, Sakakibara Y, Hase T, Grandjean O, Clément G, Citerne S, Boutet-Mercey S, Masclaux-Daubresse C, Chardon F, Soulay F, … Suzuki A. (2017). ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds. The Plant Journal, 91: 371-393. I

Cañas RA, Yesbergenova-Cuny Z, Simons M, Chardon F, Armengaud P, Quilleré I, Cukier C, Gibon Y, Limami AM, Nicolas SD, Brulé L, Lea PJ, Maranas CD, & Hirel B. (2017). Exploiting the Genetic Diversity of Maize using a Combined Metabolomic, Enzyme Activity Profiling, and Metabolic Modelling Approach to Link Leaf Physiology to Kernel Yield. Plant Cell.DOI:

Avila-Ospina L, Clément G, & Masclaux-Daubresse C. (2017). Metabolite profiling for leaf senescence in barley reveals decreases in amino acids and glycolysis intermediates. MDPI Agronomy, 7:15.

Gaufichon L, Marmagne A, Yoneyama T, Hase T, Clément G, Trassaert M, Xu X, Shakibaei M, Najihi A, & Suzuki A. (2016). Impact of the Disruption of ASN3-Encoding Asparagine Synthetase on Arabidopsis Development. MDPI Agronomy, 6: 12.

Avila-Ospina L, Marmagne A, Soulay F, & Masclaux-Daubresse C. (2016). Identification of Barley (Hordeum vulgare L.) Autophagy Genes and Their Expression Levels during Leaf Senescence, Chronic Nitrogen Limitation and in Response to Dark Exposure. MDPI Agronomy, 6, 15.

Avila-Ospina L, Marmagne A, Talbotec J, Krupinska K, & Masclaux-Daubresse C. (2015). The identification of new cytosolic glutamine synthetase and asparagine synthetase genes in barley (Hordeum vulgare L.), and their expression during leaf senescence. Journal of Experimental Botany, 66, 2013-2026.

Orsel M, Moison M, Clouet V, Thomas J, Leprince F, Canoy A-S, Just J, Chalhoub B, & Masclaux-Daubresse C. (2014). Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence. Journal of Experimental Botany, 65, 3927-3947

Merkulova EA, Guiboileau A, Naya L, Masclaux-Daubresse C, & Yoshimoto K. (2014). Assessment and Optimization of Autophagy Monitoring Methods in Arabidopsis Roots Indicate Direct Fusion of Autophagosomes with Vacuoles. Plant and Cell Physiology, 55: 715-726.

Merkulova EA, Guiboileau A, Naya L, Masclaux-Daubresse C, & Yoshimoto K. (2014). Assessment and Optimization of Autophagy Monitoring Methods in Arabidopsis Roots Indicate Direct Fusion of Autophagosomes with Vacuoles. Plant and Cell Physiology, 55: 715-726.

Masclaux-Daubresse C, Clément G, Anne P, Routaboul J, Guiboileau A, Soulay F, Shirasu K, & Yoshimoto K. (2014). Stitching together the multiple dimensions of autophagy using metabolomic and transcriptomic analyses reveals new impacts of autophagy defects on metabolism, development and plant response to environment. Plant Cell, 26, 1857-1877.

Chardon F, Jasinski S, Durandet M, Lécureuil A, Soulay F, Bedu M, Guerche P, & Masclaux-Daubresse C. (2014). QTL meta-analysis in Arabidopsis reveals an interaction between leaf senescence and resource allocation to seeds. Journal of Experimental Botany, 65: 3949-3962.

Gaufichon L, Masclaux-Daubresse C, Tcherkez G, Reisdorf-Cren M, Sakakibara Y, Hase T, Clément G, Avice J, Grandjean O, Marmagne A, Boutet-Mercey S, Azzopardi M, Soulay F, Suzuki A (2013) Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth. Plant Cell and Environment 36: 328-342

Guiboileau A, Avila-Ospina L, Yoshimoto K, Soulay F, Azzopardi M, Marmagne A, Lothier J, Masclaux-Daubresse C (2013) Physiological and metabolic consequences of autophagy defisciency for the management of nitrogen and protein resources in Arabidopsis leaves depending on nitrate availability. New Phytologist 199: 683-94.

Chardon F, Bedu M, Calenge F, Klemens PAW, Spinner L, Clement G, Chietera G, Leran S, Ferrand M, Lacombe B, Loudet O, Dinant S, Bellini C, Neuhaus HE, Daniel-Vedele F, Krapp A (2013) Leaf Fructose Content Is Controlled by the Vacuolar Transporter SWEET17 in Arabidopsis. Current Biology 23: 697-702

Guiboileau A, Masclaux-Daubresse C (2012) Autophagic processes in plants: Mechanisms, regulation and function. Comptes Rendus Biologies 335: 375-388

Yoshimoto K (2012) Beginning to Understand Autophagy, an Intracellular Self-Degradation System in Plants. Plant and Cell Physiology 53: 1355-1365

Ikram S, Bedu M, Daniel-Vedele F, Chaillou S, Chardon F (2012) Natural variation of Arabidopsis response to nitrogen availability. Journal of Experimental Botany 63: 91-105
Guiboileau A, Yoshimoto K, Soulay F, Bataillé M, Avice J, Masclaux-Daubresse C (2012) Autophagy machinery controls nitrogen remobilization at the whole-plant level under both limiting and ample nitrate conditions in Arabidopsis. New Phytologist 194: 732-740

Chardon F, Noel V, Masclaux-Daubresse C (2012) Exploring NUE in crops and in Arabidopsis ideotypes to improve yield and seed quality. Journal of Experimental Botany 63: 3401-3412

Lothier J, Gaufichon L, Sormani R, Lemaitre T, Azzopardi M, Morin H, Chardon F, Reisdorf-Cren M, Avice J-C, Masclaux-Daubresse C (2011) The cytosolic glutamine synthetase GLN1;2 plays a role in the control of plant growth and ammonium homeostasis in Arabidopsis rosettes when nitrate supply is not limiting. Journal of Experimental Botany 62: 1375-1390

Masclaux-Daubresse C, Chardon F (2011) Exploring nitrogen remobilization for seed filling using natural variation in Arabidopsis thaliana. Journal of Experimental Botany 62: 2131-2142

Sormani R, Masclaux-Daubresse C, Daniele-Vedele F, Chardon F (2011) Transcriptional regulation of ribosome components are determined by stress according to cellular compartments in Arabidopsis thaliana. PLoS ONE 6: e28070

Masclaux-Daubresse C, Daniel-Vedele F, Dechorgnat J, Chardon F, Gaufichon L, Suzuki A (2010) Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture. Annals of Botany 105: 1141-1157

Guiboileau A, Sormani R, Meyer C, Masclaux-Daubresse C (2010) Senescence and death of plant organs: Nutrient recycling and developmental regulation. Comptes Rendus Biologies 333: 382-391

Chardon F, Barthelemy J, Daniel-Vedele F, Masclaux-Daubresse C (2010) Natural variation of nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana cultivated with limiting and ample nitrogen supply. Journal of Experimental Botany 61: 2293-2302

Wingler A, Purdy SJ, Edwards S-A, Chardon F, Masclaux-Daubresse C (2010) QTL analysis for sugar-regulated leaf senescence supports flowering-dependent and -independent senescence pathways. New Phytologist 185: 420-433

Lemaitre T, Gaufichon L, Boutet-Mercey S, Christ A, Masclaux-Daubresse C (2008) Enzymatic and metabolic diagnostic of nitrogen deficiency in Arabidopsis thaliana Wassileskija accession. Plant and Cell Physiology 49: 1056-1065
Masclaux-Daubresse C, Reisdorf-Cren M, Orsel M (2008) Leaf nitrogen remobilisation for plant development and grain filling. Plant Biology 10: 23-36

Diaz C, Lemaitre T, Christ A, Azzopardi M, Kato Y, Sato F, Morot-Gaudry J-F, Le Dily F, Masclaux-Daubresse C (2008) Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition. Plant Physiology 147: 1437-1449
Masclaux-Daubresse C, Purdy S, Lemaitre T, Pourtau N, Taconnat L, Renou J-P, Wingler A (2007) Genetic variation suggests interaction between cold acclimation and metabolic regulation of leaf senescence. Plant Physiology 143: 434-446

Tavernier V, Cadiou S, Pageau K, Lauge R, Reisdorf-Cren M, Langin T, Masclaux-Daubresse C (2007) The plant nitrogen mobilization promoted by Colletotrichum lindemuthianum in Phaseolus leaves depends on fungus pathogenicity. Journal of Experimental Botany 58: 3351-3360

Masclaux-Daubresse C, Reisdorf-Cren M, Pageau K, Lelandais M, Grandjean O, Kronenberger J, Valadier MH, Feraud M, Jouglet T, Suzuki A (2006) Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco. Plant Physiology 140: 444-456

Diaz C, Saliba-Colombani V, Loudet O, Belluomo P, Moreau L, Daniel-Vedele F, Morot-Gaudry J-F, Masclaux-Daubresse C (2006) Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana. Plant & cell physiology 47: 74-83

Pageau K, Reisdorf-Cren M, Morot-Gaudry JF, Masclaux-Daubresse C (2006) The two senescence-related markers, GS1 (cytosolic glutamine synthetase) and GDH (glutamate dehydrogenase), involved in nitrogen mobilization, are differentially regulated during pathogen attack and by stress hormones and reactive oxygen species in Nicotiana tabacum L. leaves. Journal of Experimental Botany 57: 547-557

Diaz C, Purdy S, Christ A, Morot-Gaudry JF, Wingler A, Masclaux-Daubresse CL (2005) Characterization of markers to determine the extent and variability of leaf senescence in Arabidopsis. A metabolic profiling approach. Plant Physiology 138: 898-908

Masclaux-Daubresse C, Carrayol E, Valadier MH (2005) The two nitrogen mobilisation- and senescence-associated GS1 and GDH genes are controlled by C and N metabolites. Planta 221: 580-588





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