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Variation and Abiotic Stress Tolerance
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Keywords :Arabidopsis thaliana - core collection - accession - phenotype - drought - osmotic - QTL - cold tolerance - acclimation - RT-PCR

Doctoral school affiliation : ED 145 Sciences du végétal, Université ParisSud 11, Orsay

 

The VAST lab website


Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 7
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19

 

Group leader
Olivier Loudet
Senior scientist

Isabelle Gy
Engineer

Michel Burtin
Technician

Zeyun Xue
PhD student
from 1/10/16 to 30/9/20

 

 

 

 

Evelyne Téoulé
Associate Professor

Carine Géry
Assistant Engineer


Marina Ferrand
Technician


 

 

ex-lab members

 


Summary :


The group is exploiting genomic methods as well as classical and quantitative genetics to understand the genetic networks that underly complex characters such as abiotic stress responses. In order to undertake this work, the department has invested in high performance tools needed to understand genetic variation: the department has created and manages series of genetic resources for Arabidopsis thaliana, it has put in place a high-throughput genotyping platform, and it is developping a high-throughput phenotyping tool.
The study of the response to abiotic stress is of both fundamental and agronomic interest. Abiotic stress is a major cause of lower yield, for all types of crops, and in diverse environments. Plants, as organisms dependent on their surroundings, have more or less developed capacities to tolerate episodes of climatic extremes. Certain plant species or varieties within a species are capable of continued growth and reproduction in physical conditions that vary from their norms ; for this they take advantage of the plasticity of their genomes. Others restrict their life cycle in a series of narrower parameters ; they are adapted to a particular surrounding. This diversity in behavior is exploited to understand the mechanisms of tolerance to abiotic stress, in particular, drought, osmotic and cold stress. To breed crops adapted to their evolving culture conditions -a major challenge for the XXIst century- it is necessary to get a better understanding of stress signal pathways, their regulation and the mechanisms of the physiological responses.

 


Main Results :

Water deficit and osmotic stress

We are looking for variable factors (genes) that intervenes in the tolerance to these stresses. To achieve this, several approaches have been taken :

  • The identification of major and minor QTLs (Quantitative Trait Loci) will allow us to update the list of genes involved in the responses to these stresses as well as the interesting alleles and their interactions within a network of genes.
  • In parallel, association studies, relying on statistical methods, between the genotype of candidate genes and the behavior of the plant under stress, will be performed. This approach will confirm or not the importance of the candidate genes in the expression of the trait.
  • When the QTLs define new candidate genes, positional cloning of the gene(s) will be conducted. This key step will benefit from our access to the high-throughput genotyping and phenotyping platforms.

Concerning water deficit, we have determined conditions for a moderate stress to obtain an interaction between the response of the genotypes and the treatment. The plants are grown on a commercially available substrate, in sub-optimal conditions of hydration. We have determined the vegetative and reproductive traits that vary with the genotype and with the hydration conditions.
Osmotic stress is studied in vitro by the application of a moderate osmotic stress (mannitol, PEG). The effect on the growth of the leaves and the seedling is used as a model for cellular growth and the integration of signals from the environment. The QTLs detected at this stage will also be studied individually in conditions of water deficit.
Root system architecture is another important factor that needs to be studied in details to understand a genotype's response to drought stress. We use in vitro displays to do so, allowing us also to measure the response to osmotic stress.

In parallel to these quantitative genetics studies, our team is involved in a large genomics program aiming at identifying mutants in genes with unknown function, named “orphan genes”, altered in their response to drought stress. In a list of mutants that were selected in silico, a systematic screen allowed us to retain about forty mutants for deeper genetic, physiological and molecular analysis. Once genes are validated on their role in response to drought, a complete functional analysis is engaged to unravel their function.

More information on www.inra.fr/vast

 

Cold tolerance

Our team is working on mechanisms involved in cold tolerance, mainly on the model plant Arabidopsis thaliana.
Some mechanisms allowing plants to survive at freezing temperature have been partially elucidated recently. A lot of studies dealing with the acclimation process have conducted to identify some response pathway, especially the CBF one. On the other hand, transcriptome analyses suggest that other pathways exist and have to be unravelled.
Several strategies are currently being done in our team to identify such new genes.
The first one consists in exploring natural variability in order to characterize accessions exhibiting variable response to cold stress. Based on this first screen and using the genetic resources of the Centre of Resources in Versailles (mainly RIL), a QTL approach could allow identification of new genes. 8 accessions of the core collection, showing variable degree of tolerance have been characterised in this purpose.
In parallel, we have participated to a genomic project dealing with orphan genes. Mutants affected in such genes and impaired in the cold response have been searched.
At last we are studying natural variability for CBF gene family. Natural diversity is analysed by sequencing, specific gene expression and phenotypic test for cold tolerance. Combining all these different data should elucidate the importance of this pathway in the cold response in relation with genetic background and produce data on the role of each member of this gene family. Moreover RNAi approach is used to inactivate CBF genes in different accessions. Phenotypic test on these lines will complete data on previous analysis.
All these approaches need a robust phenotypic test. We have elaborated a freezing test including or not an acclimation phase. Plants are grown in soil and the test is realised in climate rooms able to freeze. This is destructive for the most sensitive plants. Damages are evaluated on a scale from o (no damage) to 6 (dead plants). (Protocol is available by asking).

observations on 2 types of seed sowing (in groups of seeds or individually) after the same cold stress.


Results


The phenotypic test is reliable, reproducible, and allows revealing high variability. It has been used to
evaluate the cold response of the 48 accessions of the core collection. Among these, 4 are very sensitive, apparently unable to acclimate, and 4 are very tolerant until –8°C after acclimation.
We didn’t identify any accessions able to survive to freezing period without acclimation as the Eskimo mutant does. Crosses between sensitive and resistant accessions indicate that resistance is mostly dominant. Crosses between sensitive lines are mostly sensitive indicating that common genes are involved. A plasmid has been constructed to induce depletion of CBF by RNAi and has been introduced in 9 different accessions. Phenotypic analysis of the transformed lines is presently realised.
Expression analysis of CBF1, CBF2 and CBF3 has been performed on accessions exhibiting contrasted responses to cold stress. A synthetic analysis of phenotypic data and expression results is under study.
In the program on orphan genes: among 225 mutant lines that have been selected in silico, a mass screening has revealed 80 interesting ones. These will be submitted to deeper genetic analyses. The best candidates will then enter a functional analysis.


Epigenetic regulations


The importance of epigenetic processes in Eucaryotic gene regulation was established in the past years, and experiments undertaken on plants played a key role in the discovery of the mechanisms underlying these processes. It is now clear that gene expression is not regulated only throughout interactions between promoters and transcriptional activators, and that it can also be modulated epigenetically via chromatin remodelling, DNA methylation, histone modifications, mRNA degradation or translational repression. Epigenetic modifications do not alter the DNA sequence, but are heritable mitotically and/or meiotically and are potentially reversible. Most epigenetic regulations depend upon DNA/RNA or RNA/RNA interactions involving small RNAs (20-25 nucleotides long).
Phenotypic changes have been observed in plants exposed to extreme environments as well as in their
progenies. This led to the hypothesis that environmental stimuli could induce heritable epigenetic modifications. The occurence of epialleles in response to environmental stresses, followed by selection, would allow a rapid adaptation to new conditions, without changes in the DNA sequence. The potentially reversible nature of epialleles allows some plasticity of gene expression. This feature would be advantageous if the inducing environmental conditions were relatively short-termed.
Our aim is to determine if epigenetic regulations have an impact on the adaptability and evolution of plant species. To do so, we will use the model plant species Arabidopsis thaliana, and will focus our research on its tolerance to cold and moderate drought.



Selected Publications :

Bazakos C., Hanemian M., Trontin C., Jiménez-Gómez J.M., Loudet O. (2017) New strategies and tools in quantitative genetics: how to go from the phenotype to the genotype. Annual Review of Plant Biology doi: 10.1146/annurev-arplant-042916-040820 (pubmed)

Agorio A., Durand S., Fiume E., Brousse C., Gy I., Simon M., Anava S., Rechavi O., Loudet O., Camilleri C., Bouché N. (2017) An Arabidopsis natural epiallele maintained by a feed-forward silencing loop between histone and DNA. PLoS Genetics 13:e1006551 (pdf)

Viaud G., Loudet O., Cournède P-H. (2017) Leaf segmentation and tracking in Arabidopsis thaliana combined to an organ-scale plant model for genotypic differentiation. Frontiers in Plant Science 7: 2057 doi: 10.3389/fpls.2016.02057 (pubmed)

Nijveen H., Ligterink W., Keurentjes J.J.B., Loudet O., Long J., Sterken M.G., Prins P., Hilhorst H.W., de Ridder D., Kammenga J.E., Snoek B.L. (2017) AraQTL - Workbench and archive for systems genetics in Arabidopsis thaliana. The Plant Journal 89: 1225-1235 (pubmed)

Shahzad Z., Canut M., Tournaire-Roux C., Martinière A., Boursiac Y., Loudet O., Maurel C. (2016) A potassium-dependent oxygen sensing pathway regulates plant root hydraulics. Cell 167: 87-98 Supp. Material (pubmed) press release INRA

Müller NA, Wijnen CL, Srinivasan A, Ryngajllo M, Ofner I, Lin T, Ranjan A, West D, Maloof JN, Sinha NR, Huang S, Zamir D, Jiménez-Gómez JM. (2016) Domestication selected for deceleration of the circadian clock in cultivated tomato. Nature Genetics 48(1): 89-93 (pubmed)

Cubillos F.A., Stegle O., Grondin C., Canut M., Tisné S., Gy I., Loudet O. (2014) Extensive cis-regulatory variation robust to environmental perturbation in A. thaliana. The Plant Cell 26(11):4298-310 (PubMed)

Schmalenbach I, Zhang L, Ryngajllo M, Jiménez-Gómez JM. (2014) Functional analysis of the Landsberg erecta allele of FRIGIDA. BMC Plant Biology 14(1): 218 (FullText, pdf)

Téoulé E., Géry C. (2014) Mapping of quantitative trait loci (QTL) associated with plant freezing tolerance and cold acclimation. Methods in Molecular Biology 1166: 43-64 (PubMed)

Gloggnitzer J, Akimcheva S, Srinivasan A, Kusenda B, Riehs N, Stampfl H, Bautor J, Dekrout B, Jonak C, Jiménez-Gómez JM, Parker JE, Riha K. (2014) Nonsense-mediated mRNA decay modulates immune receptor levels to regulate plant antibacterial defense. Cell Host & Microbe 16(3): 376-390 (PubMed)

Bolger A, Scossa F, Bolger ME, Lanz C, Maumus F, Tohge T, Quesneville H, Alseekh S, Sørensen I, Lichtenstein G, Fich EA, Conte M, Keller H, Schneeberger K, Schwacke R, Ofner I, Vrebalov J, Xu Y, Osorio S, Aflitos SA, Schijlen E, Jiménez-Goméz JM, Ryngajllo M, Kimura S, Kumar R, Koenig D, Headland LR, Maloof JN, Sinha N, van Ham RC, Lankhorst RK, Mao L, Vogel A, Arsova B, Panstruga R, Fei Z, Rose JK, Zamir D, Carrari F, Giovannoni JJ, Weigel D, Usadel B, Fernie AR. (2014) The genome of the stress-tolerant wild tomato species Solanum pennellii. Nature Genetics 46(9): 1034-1038 (FullText)

Trontin C., Kiani S., Corwin J.A., Hématy K., Yansouni J., Kliebenstein D.J., Loudet O. (2014) A pair of receptor-like kinases is responsible for natural variation in shoot growth response to mannitol treatment in Arabidopsis thaliana. The Plant Journal 78(1): 121-133 (Pubmed)

Jiménez-Gómez JM. (2014) Network types and their application in natural variation studies in plants. Current Opinion in Plant Biology 18: 80-86 (PubMed)

Saez-Aguayo S., Rondeau-Mouro C., Macquet A., Kronholm I., Ralet M.-C., Berger A., Sallé C., Poulain D., Granier F., Botran L., Loudet O., de Meaux J., Marion-Poll A., North H.M. (2014) Local evolution of seed flotation in Arabidopsis. PLoS Genetics 10(3): e1004221 FullText online

Coustham V., Vlad D., Deremetz A., Gy I., Cubillos F.A., Kerdaffrec E., Loudet O., Bouché N. (2014) SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1. PLoS ONE 9(1): e84687 FullText online/ Supp. Figures / Supp. Tables

Koprivova A., Giovannetti M., Baraniecka P., Lee B.R., Grondin C., Loudet O., Kopriva S. (2013) Natural variation in ATPS1 isoform of ATP Sulfurylase contributes to control of sulfate levels in Arabidopsis. Plant Physiology 163(3): 1133-1141 (pdf)

Chardon F., Bedu M., Calenge F., Klemens P.A.W., Spinner L., Clement G., Chietera G., Léran S., Ferrand M., Lacombe B., Loudet O., Dinant S., Bellini C., Neuhaus H.E., Daniel-Vedele F., Krapp A. (2013) Leaf fructose content is controlled by the vacuolar transporter SWEET17 in Arabidopsis. Current Biology  23(8): 697-702   Supp. Material (pdf)

Silveira A.B., Trontin C., Cortijo S., Barau J., Vieira Del Bem L.E., Loudet O., Colot V., Vincentz M. (2013) Extensive natural epigenetic variation at a de novo originated gene.  PLoS Genetics  9(4): e1003437   Paper eLink   /   Supp. Figures (pdf)

Tisné S., Serrand Y., Bach L., Gilbault E., Ben Ameur R., Balasse H., Voisin R., Bouchez D., Durand-Tardif M., Guerche P., Chareyron G., Da Rugna J., Camilleri C., Loudet O. (2013)  PHENOSCOPE: an automated large-scale phenotyping platform offering high spatial homogeneity. The Plant Journal  74: 534-544   Supp. Material (pdf)

Pineau C., Loubet S., Lefoulon C., Chalies C., Fizames C., Lacombe B., Ferrand M., Loudet O., Berthomieu P., Richard O. (2012)  Natural variation at the FRD3 MATE transporter locus reveals cross-talk between Fe homeostasis and Zn tolerance in Arabidopsis thalianaPLoS Genetics  8(12): e1003120   Paper eLink   /   Supp. Figures & Tables (pdf)

Jasinski S., Lecureuil A., Miquel M., Loudet O., Raffaele S., Froissard M., Guerche P. (2012) Natural variation in seed very long chain fatty acid content is controlled by a new isoform of KCS18 in Arabidopsis thaliana. PLoS ONE  7: e49261   Paper eLink (pdf)

Poormohammad Kiani S., Trontin C., Andreatta M., Simon M., Robert T., Salt D.E., Loudet O. (2012) Allelic heterogeneity and trade-off shape natural variation for response to soil micronutrient.  PLoS Genetics  8(7): e1002814   Paper eLink   /   Supp. Figures   /   Supp. Tables  (pdf)

Cubillos F.A., Yansouni J., Khalili H., Balzergue S., Elftieh S., Martin-Magniette M.L., Serrand Y., Lepiniec L., Baud S., Dubreucq B., Renou J.P., Camilleri C., Loudet O. (2012)  Expression variation in connected recombinant populations of Arabidopsis thaliana highlights distinct transcriptome architectures. BMC Genomics  13: 117   Supp. Text&Figures   /   Supp. Tables   /   The QTL Store  (pdf)

Routaboul J.M., Dubos C., Beck G., Marquis C., Bidzinski P., Loudet O., Lepiniec L. (2012) Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis. Journal of Experimental Botany  63: 3749-3764  Supp. Data (pdf)

Bouteillé M., Rolland G., Balsera C., Loudet O., Muller B. (2012) Disentangling the intertwined genetic bases of root and shoot growth in Arabidopsis. PLoS ONE  7, e32319 Paper eLink (pdf)

Durand S., Bouché N., Perez Strand E., Loudet O., Camilleri C. (2012) Rapid establishment of genetic incompatibility through natural duplication-mediated epigenetic variation. Current Biology  22: 326-331  Supp. Data (pdf)

Cubillos F.A., Coustham V., Loudet O. (2012) Lessons from eQTL mapping studies: non-coding regions and their role behind natural phenotypic variation in plants. Current Opinion in Plant Biology  15: 192-198 (pdf)

Simon M., Simon A., Martins F., Botran L., Tisné S., Granier F., Loudet O., Camilleri C. (2011) DNA fingerprinting and new tools for fine-scale discrimination of Arabidopsis thaliana accessions. The Plant Journal  69: 1094-1101  Supp. Fig.  /   Supp. Tab.   /   Cluster   /   ANATool  (pdf)

C. Géry, E. Zuther, E. Schulz, J. Legoupi, A. Chauveau, H. McKhann, D. K. Hincha, E. Téoulé.(2011) , Natural variation in the freezing tolerance of Arabidopsis thaliana : effects of RNAi-induced CBF depletion and QTL localisation vary  among accessions.  Plant Sci. 2011 Janv, vol. 180, Issue 1, 12-23                             

Trontin C., Tisné S., Bach L., Loudet O. (2011) What does Arabidopsis natural variation teach us (and does not teach us) about adaptation in plants? Current Opinion in Plant Biology 14: 225-231 (pdf)

Lefebvre V., Fortabat M-N., Ducamp A., North H.M., Maia-Grondard A., Trouverie J., Boursiac Y., Mouille G., Durand-Tardif M. (2011) ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. PLoS ONE 6(2): e16645 Paper eLink (pdf)

Vlad D., Rappaport F., Simon M., Loudet O. (2010) Gene transposition causing natural variation for growth in Arabidopsis thaliana. PLoS Genetics 6(5): e1000945 Paper eLink / Supp. Fig. / Supp. Tab. (pdf)

Kronholm I., Loudet O., de Meaux J. (2010) Influence of mutation rate on estimators of genetic differentiation - lessons from Arabidopsis thaliana. BMC Genetics 11:33 (pdf)

Platt A., Horton M., Huang Y.S., Li Y., Anastasio A.E., Wayan Mulyati N., Ågren J., Bossdorf O., Byers D., Donohue K., Dunning M., Holub E.B., Hudson A., Le Corre V., Loudet O., Roux F., Warthmann N., Weigel D., Rivero L., Scholl R., Nordborg M., Bergelson J., Borevitz J.O. (2010) The scale of population structure in Arabidopsis thaliana PLoS Genetics 6(2): e1000843 Paper eLink (pdf)

Lefebvre V, Poormohammad K, Durand-Tardif M (2009). A Focus on Natural Variation for Abiotic Constraints Response in the Model Species Arabidopsis thaliana. J Mol. Sci, 10 : 3547-3582 (pdf)

Bikard D, Patel D, Le Metté C, Giorgi V, Camilleri C, Bennett M.J, Loudet O (2009). Divergent evolution of duplicate genes leads to genetic incompatibilities within A. thaliana. Science, 323: 623-626 Supp. Data / High Res. Figures / Free Full Text.(pdf)

Balasubramanian S, Schwartz C, Singh A, Warthmann N, Kim MC, Maloof JN, Loudet O, Trainer GT, Dabi T, Borevitz JO, Chory J, Weigel D (2009). QTL Mapping in new Arabidopsis thaliana advanced intercross-recombinant inbred lines. PLoS ONE, 4 (2) : e4318 (on-line) (pdf)

Bouchabke-Coussa O, Quashie ML, Seoane-Redondo J, Fortabat MN, Gery C, Yu A, Linderme D, Trouverie J, Granier F, Téoulé E, Durand-Tardif M.(2008) ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance. BMC Plant Biol. 2008 Dec 7;8:125

McKhann HI, Gery C, Bérard A, Lévêque S, Zuther E, Hincha DK, De Mita S, Brunel D, Téoulé E.(2008) . Natural variation in CBF gene sequence, gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana. BMC Plant Biol. 2008 Oct 15;8:105

Bouchabke-Coussa O, Quashie M.-L., Seoane-Redondo J., Fortabat M.-N., Gery C., Yu A., Linderme D., Trouverie J., Granier F., Teoule E. and M. Durand-Tardif (2008). Is a key gene involved in water economy as well as cold acclimation and salt tolerance, BMC Plant Biology 8:125 (pdf)

Ainsworth EA, Beier C, Calfapietra C, Ceulemans R, Durand-TardifnM, Ainsworth EA, Beie C, Calfapietra C, Ceulemans R, Durand-Tardif M, Farquhar GD, Godbold DL, Hendrey GR, Hickler T, Kaduk J, Karnosky DF, Kimball BA, Korner C, Koornneef M, Lafarge T, Leakey A D, Lewin K F, Long.S P, Manderscheid R, McNeil D L, Mies T A, Miglietta.F, Morgan.J.A, Nagy.J, Norby.R.J, Norton.R.M, Percy.K.E, Rogers.A, Soussana.J. F, Stitt.M, Weigel.H. J, White JW (2008). Next generation of elevated [CO(2)] experiments with crops: A critical investment for feeding the future world. Plant Cell Environ, 31 : 1317-1324.(pdf)

Loudet O, Michael TP, Burger BT, Le MettéC, Mockler TC, Weigel D, Chory J (2008). A zinc knuckle protein that negatively controls morning-specific growth in Arabidopsis thaliana. PNAS, In press.

Meng PH, Macquet A, Loudet O, Marion-Poll A, North HM (2008). Analysis of natural allelic variation controlling Arabidopsis thaliana seed germinability in response to cold and dark: identification of three major quantitative trait loci. Mol Plant, 1 145-154.

Sicard O, Loudet O, Keurentjes J.J, Candresse T, Le Gall O, Revers F, Decroocq V (2008). Mol Plant Microbe Interact 21(2) 198-207.

Bouchabke O, Chang F, Simon M, Voisin R, Pelletier G, Durand-Tardif M (2007). Natural variation in Arabidopsis thaliana as a tool for highlighting differential drought responses. Accepted with revisions in PloS-One Nov. (pdf)

Loudet O, Saliba-Colombani V, Camilleri C, Calenge F., Gaudon V, Koprivova A, North KA, Kopriva S., Daniel-Vedele F (2007). Natural variation for sulfate content in Arabidopsis is highly controlled by APR2 . Nature Genetics 39 : 896-900 (pdf)

Macquet A, Ralet MC, Loudet O, Kronenberger J, Mouille G, Marion-Poll A, North H.M. (2007). A naturally occurring mutation in an Arabidopsis accession affects a beta-D-galactosidase that increases the hydrophilic potential of rhamnogalacturonan I in seed mucilage. Plant Cell 19(12) 3990-4006.

Perchepied L, Kroj T, Tronchet M, Loudet O, Roby D (2006). Natural variation in partial resistance to Pseudomonas syringae is controlled by two major QTLs in Arabidopsis thaliana. PLoS ONE 1(1): e123 (on-line) (pdf)

Calenge F., Saliba-Colombani V., Mahieu S., Loudet O., Daniel-Vedele F., Krapp A. (2006). Natural variation for carbohydrate content in Arabidopsis thaliana: interaction with complex traits dissected by quantitative genetics.
Plant Physiol, June 23 Epub -(pdf)

Kliebenstein D.J., West M.A.L., van Leeuwen H., Loudet O., Doerge R.W., St.Clair D.A. (2006). Identification of QTLs controlling gene expression networks defined a priori. MC Bioinformatics, June 16 Epub - pdf

Mouille G, Witucka-Wall H, Bruyant M.P, Loudet O, Rihouey C, Lerouxel O, Lerouge P, Hofte H, Pauly M (2006).
QTL analysis of primary cell wall composition in Arabidopsis thaliana. Plant Physiol, 141: 1035-1044 - pdf

Diaz C, Saliba-Colombani V, Loudet O., Belluomo P, Moreau L, Daniel-Vedele F, Morot-Gaudry J.-F, Masclaux-Daubresse C (2006). Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana. Plant Cell Physiol, 47 : 74-83 6. (pdf)

Reymond M, Svistoonoff S, Loudet O, Nussaume L, Desnos T (2006). Identification of QTL controlling root growth response to phosphate starvation in Arabidopsis thaliana. Plant Cel Env, 29 : 115-125.(pdf)

Y. Barrière, D. Denoue, M. Briand, M. Simon, L. Jouanin and M. Durand-Tardif (2006). Genetic variation for cell walldigestibility related traits in floral stems of A. thaliana accessions as a basis for the improvement of the feeding value in maize and forage plants, Theor. Appl. Genet, 113 : 163-175.(pdf)

Loudet O, Gaudon V, Trubuil A, Daniel-Vedele F (2005). Quantitative trait loci controlling root growth and architecture in Arabidopsis thaliana confirmed by heterogeneous inbred family. Theoretical Applied Genet, 110: 742-753. (PubMed) (pdf)

C ; Menanad, B ; Lei, Y ; Sormani, R ; Nicolai, M ; Géry, C ; Téoulé, E ;Deprost, D ; Meyer, (2004) C. Biochemical Society Transactions, Plant growth : the translational connection. Robaglia, 32 : 581-584 Part 4 AUG 2004.

Loudet O, Chaillou S, Merigout P, Talbotec J, Daniel-Vedele F (2003). Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis. Plant Physiol,131 : 345-358. (PubMed) (pdf)

Loudet O, Chaillou S, Krapp A, Daniel-Vedele F (2003). Quantitative trait loci analysis of water and anion contents in interaction with nitrogen availability in Arabidopsis thaliana. Genetics. 163 : 711-22. (PubMed) (pdf)

Loudet O, Chaillou S, Camilleri C, Bouchez D, Daniel-Vedele F (2002). Bay-0 x Shahdara recombinant inbred line population: a powerful tool for the genetic dissection of complex traits in Arabidopsis. Theor Appl Genet, 104 : 1173-1184. (PubMed) - PDF

 

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