We
have designed and set up a robotic platform for automated culture
and measurement of Arabidopsis plants in a fully controlled
environment.
A prototype was made in collaboration with ISEP
(Institut Supérieur d'Electronique de Paris) and CEGS-DESTEC.
Each robot is placed in a growth chamber and can accomodate up to
750 individual Arabidopsis plants. Homogeneity is achieved
thanks to constant rotation of plants on the device. Each plant
is weighed and adjusted for water and minerals every 4 hours, according
to user-defined individual parameters. A measure station is equipped
with sensors (visible, infrared or fluorescence imaging) for evaluation
of developmental and physiological variables in response to imposed
constraints.
Moreover,
the IJPB, thanks to financial support from the Région
Ile de France, INRA
and Génoplante,
is building a new facility for 300 m2 of culture chambers, which
will eventually accomodate the IJPB high throughput phenotyping
platform. A dedicated chamber of 60m2, fully automated, will allow
experiments with more than 10,000 individuals in parallel.
|