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Epigenetics and small RNAs
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Keywords : Arabidopsis thaliana, Mutant, Chromatin, Methylation, RNA interference, siRNA, miRNA, Development, Virus

Doctoral school affiliation : ED 567 Sciences du Végétal

Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech-ERL3559 CNRS
Bâtiment 2

INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19


Group Leader
Hervé Vaucheret
Senior Scientist

Associate Group Leader
Taline Elmayan
Research Scientist

Filipe Borges
Research Scientist

Chouaib Meziadi

Ivan Le Masson

Agnès Yu

Nathalie Bouteiller

Hayat Sehki
PhD Student

Summary :

TGS du gene GUS Eukaryotes regulate gene expression through epigenetic mechanisms involving 21-24 nucleotides small RNAs (siRNAs and miRNAs) that lead either to transcriptional inhibition (transcriptional gene silencing: TGS), RNA degradation (post-transcriptional gene silencing: PTGS) or translational repression. chlorose due a la PTGS de la nitrite reductase Targets of these processes include exogenous pathogens (viruses, bacteria) as well as endogenous elements (genes, transposons, heterochromatin). A molecular genetic approach is used to identify the cellular genes controlling these processes and to understand the underlying mechanisms.

Main Results :

chlorose due a la PTGS de la nitrite reductase We have identified several cellular proteins involved in various silencing networks: transgene TGS and PTGS, attenuation of viral infection, post-transcriptional regulation of endogenous genes by microRNAs (miRNAs) or trans-acting short interfering RNAs (ta-siRNAs), transcriptional control of repeated elements. Beside their role in regulating development, some miRNAs and ta-siRNAs feedback regulate their own pathways in order to maintain the homeostasis of these complex regulatory networks.


Selected Publications :

Charbonnel C, Niazi AK, Elvira-Matelot E, Zytnicki M, de Bures A, Jobet E, Opsomer A, Carapito C, Shamandi N, Reichheld JP, Vaucheret H, Sáez-Vásquez J. (2017) A conserved cysteine in the dsRBD of siRNA suppressor RTL1 is essential for RNase III activity. Nucleic Acids Res 45, 11891-11907

Taochy, C, Gursanscky, NR, Cao J, Fletcher SJ, Dressel U, Mitter N, Tucker MR, Koltunow AMG, Bowman JL, Vaucheret H, Carroll BJ. (2017) A genetic screen for impaired systemic RNAi in Arabidopsis highlights the crucial role of DCL2. Plant Physiol 175, 1424-1437

Wu YY, Hou BH, Lee WC, Lu SH, Yang CJ, Vaucheret H, and Chen HM. (2017). DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation. Plant J. 90(6):1064-1078 (Pubmed)

Butel N, Le Masson I, Bouteiller N, Vaucheret H, and Elmayan T. (2017). sgs1: a neomorphic nac52 allele impairing post-transcriptional gene silencing through SGS3 downregulation. Plant J. 90(3):505-519 (Pubmed)

Elvira-Matelot, E., Hachet, M., Shamandi, N., Comella, P., Saez-Vasquez, J., Zytnicki, M., and Vaucheret, H. (2016a). Arabidopsis RNASE THREE LIKE2 modulates the expression of protein-coding genes via 24-nt siRNA-directed DNA methylation. Plant Cell 28(2):406-25 (Pubmed)

Elvira-Matelot, E., Bardou, F., Ariel, F., Jauvion, V., Bouteiller, N., Le Masson, I., Cao, J., Crespi, M.D., and Vaucheret, H. (2016b). The nuclear ribonucleoprotein SmD1 interplays with splicing, RNA quality control and post-transcriptional gene silencing in Arabidopsis. Plant Cell 28(2):426-38 (abstract)

Hematy, K., Bellec, Y., Podicheti, R., Bouteiller, N., Anne, P., Morineau, C., Haslam, R.P., Beaudoin, F., Napier, J.A., Mockaitis, K., Gagliardi, D., Vaucheret, H., Lange, H., and Faure, J.D. (2016). The Zinc-Finger Protein SOP1 Is Required for a Subset of the Nuclear Exosome Functions in Arabidopsis. PLoS Genet 12, e1005817. (online)

Shamandi N., Zytnicki M., Charbonnel C., Elvira-Matelot E., Bochnakian A., Comella P., Mallory A.C., Lepère G., Sáez-Vásquez J., Vaucheret H. (2015) Plants encode a general siRNA suppressor that is induced and suppressed by viruses. PLoS Biology 13(12):e1002326. doi: 10.1371/journal.pbio.1002326 (online) communiqué de presse INRA, 23/12/16

Parent, J.S., Jauvion, V., Bouche, N., Beclin, C., Hachet, M., Zytnicki, M. and Vaucheret, H. (2015) Post-transcriptional gene silencing triggered by sense transgenes involves uncapped antisense RNA and differs from silencing intentionally triggered by antisense transgenes. Nucleic Acids Res, 43:8464-75 (Pubmed)

Parent, J.S., Bouteiller, N., Elmayan, T. and Vaucheret, H. (2015) Respective contributions of Arabidopsis DCL2 and DCL4 to RNA silencing. Plant J, 81, 223-232 (PubMed)

Casacuberta, J.M., Devos, Y., du Jardin, P., Ramon, M., Vaucheret, H. and Nogue, F. (2015) Biotechnological uses of RNAi in plants: risk assessment considerations. Trends Biotechnol, 33, 145-147 (PubMed)

Martinez de Alba, A.E., Moreno, A.B., Gabriel, M., Mallory, A.C., Christ, A., Bounon, R., Balzergue, S., Aubourg, S., Gautheret, D., Crespi, M.D., Vaucheret, H. and Maizel, A. (2015) In plants, decapping prevents RDR6-dependent production of small interfering RNAs from endogenous mRNAs. Nucleic Acids Res, 43, 2902-2913 (Full text)

Vaucheret, H. (2015) Mode of miRNA biogenesis matters. Nature Plants, 1, 15019
Yu, A., Saudemont, B., Bouteiller, N., Elvira-Matelot, E., Lepere, G., Parent, J.S., Morel, J.B., Cao, J., Elmayan, T. and Vaucheret, H. (2015) Second site mutagenesis of a hypomorphic ago1 allele identifies SKI3 as an endogenous suppressor of PTGS. Plant Physiol. 169:1266-74

Lange, H., Zuber, H., Sement, F.M., Chicher, J., Kuhn, L., Hammann, P., Brunaud, V., Berard, C., Bouteiller, N., Balzergue, S., Aubourg, S., Martin-Magniette, M.L., Vaucheret, H. and Gagliardi, D. (2014) The RNA helicases AtMTR4 and HEN2 target specific subsets of nuclear transcripts for degradation by the nuclear exosome in Arabidopsis thaliana. PLoS Genet, 10, e1004564 (online)

Martinez de Alba, A.E., Elvira-Matelot, E. and Vaucheret, H. (2013) Gene silencing in plants: a diversity of pathways. Biochim Biophys Acta, 1829, 1300-1308 (PubMed)

Romon, M., Soustre-Gacougnolle, I., Schmitt, C., Perrin, M., Burdloff, Y., Chevalier, E., Mutterer, J., Himber, C., Zervudacki, J., Montavon, T., Zimmermann, A., Elmayan, T., Vaucheret, H., Dunoyer, P. and Masson, J.E. (2013) RNA silencing is resistant to low-temperature in grapevine. PLoS One, 8, e82652 (online)

Poulsen, C., Vaucheret, H. and Brodersen, P. (2013) Lessons on RNA silencing mechanisms in plants from eukaryotic argonaute structures. Plant Cell 25, 22-37. (pdf)

Martinez de Alba, A.E., Parent, J.S. and Vaucheret, H. (2013) Small RNA-mediated control of development in plants. in Epigenetic Memory and Control in Plants. Grafi, G. and Ohad, N. editors. Signaling and Communication in Plants 18, DOI 10.1007/978-3-642-35227-0_9. Springer-Verlag Berlin Heidelberg, 177-189.

Moreno, A.B., Martinez de Alba, A.E., Bardou, F., Crespi, M.D., Vaucheret, H., Maizel, A. and Mallory, A.C. (2013) Cytoplasmic and nuclear quality control and turnover of single-stranded RNA modulate post-transcriptional gene silencing in plants. Nucleic Acids Res 41, 4699-4708.

Vaucheret, H. and Masson, J. (2013) Les plantes aussi se soignent aux petits ARN. Biofutur 340, 39.

Zhong, S.H., Liu, J.Z., Jin, H., Lin, L., Li, Q., Chen, Y., Yuan, Y.X., Wang, Z.Y., Huang, H., Qi, Y.J. et al. (2013) Warm temperatures induce transgenerational epigenetic release of RNA silencing by inhibiting siRNA biogenesis in Arabidopsis. Proc Natl Acad Sci U S A  110, 9171-9176.

Jauvion, V., Rivard, M., Bouteiller, N., Elmayan, T. and Vaucheret, H. (2012) RDR2 partially antagonizes the production of RDR6-dependent siRNA in sense transgene-mediated PTGS. PLoS One 7, e29785.

Jouannet, V., Moreno, A.B., Elmayan, T., Vaucheret, H., Crespi, M.D. and Maizel, A. (2012) Cytoplasmic Arabidopsis AGO7 accumulates in membrane-associated siRNA bodies and is required for ta-siRNA biogenesis. EMBO J 31, 1704-1713. (Pubmed)

Le Masson, I., Jauvion, V., Bouteiller, N., Rivard, M., Elmayan, T. and Vaucheret, H. (2012) Mutations in the Arabidopsis H3K4me2/3 demethylase JMJ14 suppress posttranscriptional gene silencing by decreasing transgene transcription. Plant Cell 24, 3603-3612. (Pubmed)

Martinez de Alba, A.E., Jauvion, V., Mallory, A.C., Bouteiller, N. and Vaucheret, H. (2011) The miRNA pathway limits AGO1 availability during siRNA-mediated PTGS defense against exogenous RNA. Nucleic Acids Res 39, 9339-9344. (PubMed)

Maunoury, N., and Vaucheret, H. (2011). AGO1 and AGO2 Act Redundantly in miR408-Mediated Plantacyanin Regulation. PloS one 6, e28729.

Parent, J.S., Martinez de Alba, A.E. and Vaucheret, H. (2012) The origin and effect of small RNA signaling in plants. Front Plant Sci 3, 179. (Pubmed)

Vaucheret, H. and Chupeau, Y. (2012) Ingested plant miRNAs regulate gene expression in animals. Cell Res 22, 3-5.

Hirsch, J., Misson, J., Crisp, P.A., David, P., Bayle, V., Estavillo, G.M., Javot, H., Chiarenza, S.,Mallory, A.C., Maizel, A. et al. (2011) A novel fry1 allele reveals the existence of a mutant phenotype unrelated to 5'->3' exoribonuclease (XRN) activities in Arabidopsis thaliana roots. PLoS One 6, e16724 (pdf)

Jauvion, V., Mallory, A. and Vaucheret, H. (2011) Deciphering post-transcriptional gene silencing pathways through genetic screens. . In RNAi technology. Gaur, R.K., Gafni, Y., Sharma, P., and Gupta, V.K. editors, CRC press. Science publishers, 17-46.

Pelissier, T., Clavel, M., Chaparro, C., Pouch-Pelissier, M.N., Vaucheret, H. and Deragon, J.M. (2011) Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis. RNA 17, 1502-1510. (PubMed)

Vaucheret, H. (2011) 2001, l’odyssée des petits ARN. Biofutur 30, 32-34.

Wang, X.B., Jovel, J., Udomporn, P., Wang, Y., Wu, Q., Li, W.X., Gasciolli, V., Vaucheret, H. and Ding, S.W. (2011) The 21-nucleotide, but not 22-nucleotide, viral secondary small interfering RNAs direct potent antiviral defense by two cooperative argonautes in Arabidopsis thaliana. Plant Cell 23, 1625-1638. (PubMed)

Mallory A, Vaucheret H. (2010) Form, Function, and Regulation of ARGONAUTE Proteins. Plant Cell 22: 3879-3889. [Epub ahead of print]

Jauvion V, Elmayan T, Vaucheret H. (2010) The conserved RNA trafficking proteins HPR1 and TEX1 are involved in the production of endogenous and exogenous small interfering RNA in Arabidopsis. Plant Cell 22: 2697-709. Epub 2010 Aug 26.

Yu B, Bi L, Zhai J, Agarwal M, Li S, Wu Q, Ding SW, Meyers BC, Vaucheret H, Chen X. (2010) siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. Nucleic Acids Res. 2010 Sep 1;38(17):5844-5850. Epub 2010 May 6. PMID: 20448024 [PubMed - as supplied by publisher]Free PMC ArticleFree text

Marin E, Jouannet V, Herz A, Lokerse AS, Weijers D, Vaucheret H, Nussaume L, Crespi MD, Maizel A. (2010) miR390, Arabidopsis TAS3 tasiRNAs, and their AUXIN RESPONSE FACTOR targets define an autoregulatory network quantitatively regulating lateral root growth.  Plant Cell 22:1104-17. Epub 2010 Apr 2. PMID: 20363771 [PubMed - indexed for MEDLINE]

Mallory, A.C., Hinze, A. Tucker, M., Bouche, N., Gasciolli, V., Elmayan, T., Lauressergues, D., Jauvion, V., Vaucheret, H. and Laux, T.1 (2009) Redundant and specific roles of the ARGONAUTE proteins AGO1 and ZLL in development and small RNA-directed gene silencing. PloS Genetics 5, e1000646. doi:10.1371/journal.pgen.1000646 (PubMed)

Vaucheret H (2009) AGO1 Homeostasis Involves Differential Production of 21-nt and 22-nt miR168 Species by MIR168a and MIR168b. PLoS ONE 4(7):e6442. doi:10.1371/journal.pone.0006442 (PubMed)

Mallory AC, Vaucheret H (2009) ARGONAUTE 1 homeostasis invokes the coordinate action of the microRNA and siRNA pathways. EMBO Rep 10: 521-526.(PubMed)

Elmayan T, Adenot X, Gissot L, Lauressergues D, Gy I, et al. (2009) A neomorphic sgs3 allele stabilizing miRNA cleavage products reveals that SGS3 acts as a homodimer. FEBS J 276: 835-844.(PubMed)

Ben Amor B, Wirth S, Merchan F, Laporte P, d'Aubenton-Carafa Y, et al. (2009) Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses. Genome Res 19: 57-69.( PubMed)

Meyers BC, Axtell MJ, Bartel B, Bartel DP, Baulcombe D, et al. (2008) Criteria for annotation of plant MicroRNAs. Plant Cell 20: 3186-3190.( PubMed)

Mallory, A.C., Elmayan, T. and Vaucheret, H. (2008) MicroRNA maturation and action--the expanding roles of ARGONAUTEs. Curr Opin Plant Biol, 11, 560-566.( PubMed)

Pouch-Pelissier, M.N., Pelissier, T., Elmayan, T., Vaucheret, H., Boko, D., Jantsch, M.F., and Deragon, J.M. (2008). SINE RNA induces severe developmental defects in Arabidopsis thaliana and interacts with HYL1 (DRB1), a key member of the DCL1 complex. PLoS Genet( PubMed)

Vaucheret, H. (2008). Plant ARGONAUTES. Trends Plant Sci 13, 350-358(PubMed)

Mallory, A.C., and Bouche, N. (2008). MicroRNA-directed regulation: to cleave or not to cleave. Trends Plant Sci 13, 359-367.(PubMed)

Daxinger L, Hunter B, Sheikh M, Jauvion V, Gasciolli V, Vaucheret H, Matzke M, Furner I (2008) Unexpected silencing effects from T-DNA tags in Arabidopsis. Trends in Plant Science 13, 4-7 (PubMed)

II Gy, V Gasciolli, D Lauressergues, J-B Morel, J Gombert, F Proux, C Proux, H Vaucheret, and AC. Mallory (2007) Plant Cell Arabidopsis FIERY1, XRN2, and XRN3 Are Endogenous RNA Silencing Suppressors 10.1105/tpc.107.055319 (PubMed) and free download

Mourrain P, van Blockland R, Kooter J, Vaucheret H (2007) A single transgene locus triggers both transcriptional and post-transcriptional silencing through double-stranded RNA production. Planta 225, 365-79 (PubMed)

Rajagopalan R, Vaucheret H, Trejo J, Bartel DP (2006) A diverse and evolutionarily fluid set of miRNAs in Arabidopsis thaliana. Genes Dev, 20, 3407-25 (PubMed)

Bouché, Lauressergues, Gasciolli,Vaucheret (2006) An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs. EMBO Journal, 25, 3347-56 (PubMed)

Mallory, Vaucheret (2006) Functions of plant microRNAs and related small RNAs. Nature Genetics 38, S31-S36 (PubMed)

Adenot, Elmayan, Lauressergues, Boutet, Bouché, Gasciolli, Vaucheret (2006) DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7. Current Biology 16, 927-932 (PubMed)

Vaucheret (2006) Posttranscriptional small RNA pathways in plants: mechanisms and regulations. Genes & Development 20, 759-771 (PubMed)

Vaucheret, Mallory, Bartel (2006) AGO1 homeostasis entails co-expression of MIR168 and AGO1 and preferential stabilization of miR168 by AGO1. Molecular Cell 22, 129-136 (PubMed)

Elmayan, Proux, Vaucheret (2005) Arabidopsis RPA2: a genetic link between transcriptional gene silencing, DNA repair and DNA replication. Curr Biol 15, 1919-1925 (PubMed)

Vaucheret (2005) MicroRNA-dependent trans-acting siRNA production. Science STKE 2005, pe43 (PubMed)

Gasciolli*, Mallory*, Bartel, Vaucheret (2005) Partially redundant functions of Arabidopsis Dicer-like enzymes and a role for DCL4 in producing trans-acting siRNAs. Curr Biol 15, 1494-1500 (PubMed)

Vaucheret (2005) RNA polymerase IV and transcriptional silencing. Nat Gen 37, 659-660 (PubMed)

Park, Wu, Gonzalez-Sulser, Vaucheret, Poethig (2005) Nuclear processing and export of miRNAs in Arabidopsis. Proc. Natl. Acad. Sci. USA 102, 3691-3696. (PubMed)

Rocha, Sheikh, Melchiorre, Fagard, Boutet, Loach, Moffatt, Wagner, Vaucheret, Furner (2005) The Arabidopsis HOG1 gene codes for a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing. Plant Cell 17, 404-415 (PubMed)

Muangsan, Beclin, Vaucheret, Robertson (2004) Genetic requirements for geminivirus-mediated VIGS of endogenous genes define a new branch in the plant silencing pathway. Plant J 38, 1004-1014 (PubMed)

Vazquez*, Vaucheret*, Rajagopalan, Lepers, Gasciolli, Mallory, Hilbert, Bartel, Crété (2004) Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs. Mol Cell 16, 69-79 (PubMed)

Mallory, Vaucheret (2004) MicroRNAs: something important between the genes. Curr Opi Plant Biol 7, 120-125 (PubMed)

Vazquez, Gasciolli, Crété, Vaucheret (2004) The nuclear double-stranded RNA-binding protein HYL1 is required for proper microRNA accumulation and plant development but not for post-transcriptional transgene silencing. Curr Biol 14, 346-351 (PubMed)

Probst, Fagard, Proux, Mourrain, Boutet, Murfett, Furner, Vaucheret, Mittelstein-Scheid (2004) Arabidopsis histone deacetylase HDA6 is required for maintenance of transcriptional gene silencing and determines DNA methylation and nuclear organization of rDNA repeats. PlantCell 16, 1021-1034 (PubMed)

Vaucheret, Vazquez, Crété, Bartel (2004)The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev 18, 1187-97 (PubMed)

Boutet, Vazquez, Liu, Beclin, Fagard, Gratias, Morel, Crété, Chen, Vaucheret(2003) Arabidopsis HEN1 : a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Curr Biol 13, 843-848 (PubMed)

Morel, Godon, Mourrain, Béclin, Boutet, Feuerbach, Proux, Vaucheret (2002) Fertile hypomorphic ARGONAUTE (ago1) mutants impaired in post-transcriptional gene silencing and virus resistance. Plant Cell 14, 629-639 (PubMed)

Béclin, Boutet, Waterhouse, Vaucheret (2002) A branched pathway for transgene-induced RNA silencing in plants. Curr Biol 12, 684-688 (PubMed)

Mlotshwa, Voinnet, Mette, Matzke, Vaucheret, Ding, Pruss, Vance (2002) RNA silencing and the mobile signal. Plant Cell S289-S301(PubMed)

Vaucheret, Fagard (2001) Transcriptional gene silencing in plants: targets, inducers and regulators. Trends in Genet 17, 29-35 (PubMed)

Mallory, Ely, Smith, Marathe, Anandalakshmi, Fagard, Vaucheret, Pruss, Bowman, Vance (2001) HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal . Plant Cell 13, 571-583 (PubMed)

Vance, Vaucheret (2001) RNA silencing in plants - Defense and counterdefense. Science 292, 2277-2280 (PubMed)

Vaucheret, Fagard (2001) Transcriptional gene silencing in plants: targets, inducers and regulators. Trends Genet 17, 29-35

Chandler, Vaucheret (2001) Gene activation and gene silencing. Plant Physiol 125, 145-148

Béclin, Vaucheret (2001) L'inactivation épigénétique post-transcriptionnelle chez les végétaux: un mécanisme de résistace aux virus. Médecine/Sciences 17, 845-855

Vaucheret, Béclin, Fagard (2001) Post-transcriptional gene silencing in plants. J Cell Sci 114, 3083-3091

Mourrain, Béclin, Elmayan, Feuerbach, Godon, Morel, Jouette, Lacombe, Nikic, Picault, Rémoué, Sanial, Vo, Vaucheret. (2000) Arabidopsis SGS2 and SGS3 genes required for post-transcriptional gene silencing and virus resistance. Cell 101, 533-542 (PubMed)

Fagard, Boutet, Morel, Bellini, Vaucheret (2000) AGO1, QDE-2 and RDE-1 encode related proteins required for post-transcriptional gene silencing in plants, quelling in fungi and RNA interference in animals. Proc. Natl. Acad. Sci. USA 97, 11650-11654 (PubMed)

Marathe, Smith, Anandalakshmi, Pruss, Bowman, Fagard, Mourrain, Vaucheret, Vance (2000) Plant viral suppressors of post-transcriptional gene silencing do not suppress transcriptional gene silencing. Plant J 22, 51-59

Morel, Vaucheret (2000) Post-transcriptional gene silencing mutants. Plant Mol Biol 43, 275-284

Fagard, Vaucheret (2000) Systemic silencing signal(s). Plant Mol Biol 43, 285-293

Morel, Mourrain, Béclin, Vaucheret (2000) DNA methylation and chromatin structure affect transcriptional and post- transcriptional transgene silencing in Arabidopsis. Curr Biol 10, 1591-1594 (PubMed)

Crété, Vaucheret (1999) Expression and sequence requirements for nitrite reductase co-suppression. Plant Mol Biol 41, 105-114

Palauqui, Vaucheret (1998) Transgenes are dispensable for the RNA degradation step of co-suppression. Proc Natl Acad Sci USA 95, 9675-9680 (PubMed)

Elmayan, Balzergue, Béon, Bourdon, Daubremet, Guénet, Mourrain, Palauqui, Vernhettes, Vialle, Wostrikoff,  Vaucheret (1998) Arabidopsis mutants impaired in cosuppression. Plant Cell 10, 1747-1758 (PubMed)

Diéguez, Vaucheret, Mittelsten-Scheid, Paszkowski (1998) Cytosine methylation at CG and CNG sites is not a prerequisite for transcriptional gene silencing. Mol Gen Genet 259, 207-215

Vaucheret, Elmayan, Thierry, Van der Geest, Hall, Conner, Mlynarova, Nap (1998) Surrounding chicken, bean, yeast or tobacco matrix attachment regions (MARs) do not prevent homology-dependent trans-silencing in transgenic tobacco plants. Mol Gen Genet 259, 388-392

Béclin, Berthomé, Palauqui, Tepfer, Vaucheret (1998) Infection of tobacco or Arabidopsis plants by CMV counteracts systemic post-transcriptional silencing of non-viral (trans)genes. Virology 252, 313-317

Vaucheret, Béclin, Elmayan, Feuerbach, Godon, Morel, Mourrain, Palauqui, Vernhettes (1998) Transgene-induced gene silencing in plants.  Plant J 16, 651-659

Vaucheret, Nussaume, Palauqui, Quilléré, Elmayan (1997) A transcriptionally active state is required for post-transcriptional silencing (co-suppression) of nitrate reductase host genes and transgenes. Plant Cell 9, 1495-1504 (PubMed)

Palauqui, Elmayan, Pollien, Vaucheret (1997) Systemic Acquired Silencing: Transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J 16, 4738-4745 (PubMed)

English, Davenport, Elmayan, Vaucheret, Baulcombe (1997) Requirement of transcription for homology-dependent virus resistance and transinactivation. Plant J 12, 597-603.

Vaucheret, Palauqui, Mourrain, Elmayan (1997) Nitrate reductase and nitrite reductase as targets to study gene silencing phenomena in transgenic plants. Euphytica 93, 195-200

Park, Papp, Moscone, Iglesias, Vaucheret, Matzke, Matzke (1996) Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity. Plant J 9, 183-194

Hervieu, Vaucheret (1996) A single aminoacid change in acetolactate synthase confers resitance to valine in tobacco. Mol Gen Genet 251, 220-224

Elmayan, Vaucheret (1996) Single copies of a strongly-expressed 35S-driven transgene undergo post-transcriptional silencing. Plant J  9, 787-797

Palauqui, Elmayan, Dorlhac de Borne, Crété, Charles, Vaucheret (1996) Frequencies, timing and spatial patterns of co-suppression of nitrate reductase and nitrite reductase in transgenic tobacco plants. Plant Physiol 112, 1447-1456.

Thierry, Vaucheret (1996) Sequence homology requirements for transcriptional silencing of 35S transgenes and post-transcriptional silencing of nitrite reductase (trans)genes by the tobacco 271 locus. Plant Mol Biol 32, 1075-1083.

Vaucheret, Elmayan, Mourrain, Palauqui (1996) Analysis of a tobacco transgene locus that triggers both transcriptional and post-transcriptional silencing. Ed V.E. Russo, R.A. Martienssen, A.D. Riggs. Cold Spring Harbor Laboratory Press, pp 403-414

Vaucheret, Caboche (1995) Induction of nitrate reductase host gene expression has a negative effect on the expression of transgenes driven the nitrate reductase promoter. Plant Science 107, 95-104

Vaucheret, Palauqui, Elmayan, Moffatt (1995) Molecular and genetic analysis of nitrite reductase co-suppression in transgenic tobacco plants. Mol Gen Genet 248, 311-317

Palauqui, Vaucheret (1995) Field trial analysis of nitrate reductase co-suppression: a comparative study of 38 combinations of transgene loci. Plant Mol Biol 29, 149-159

Vaucheret, Mourrain,.Robalo, Pollien (1995) Nitrite reductase silencing as a tool for selecting spontaneous haploid plants. Plant Cell Reports 15, 12-16

Foyer, Lescure, Lefebvre, Vincentz, Vaucheret (1994) Adaptations of photosynthetic electron transport, carbon assimilation and carbon partitioning in transgenic Nicotiana plumbaginifolia plants to changes in nitrate reductase activity. Plant Physiol 104, 171-178 

Dorlhac de Borne, Vincentz, Chupeau, Vaucheret (1994) Co-suppression of nitrate reductase transgene and host genes in transgenic tobacco plants. Mol Gen Genet 243, 613-621

Vaucheret (1994) Promoter-dependant trans-inactivation in transgenic tobacco: kinetic aspects of gene silencing and gene reactivation. C R Acad Sci Paris, Life Sciences 317, 310-323

Kronenberger, Lépingle, Caboche, Vaucheret (1993) Cloning and expression of distincts nitrite reductases in tobacco leaves and roots. Mol Gen Genet  236, 203-208
Vincentz, Moureaux, Leydecker, Vaucheret, Caboche (1993) Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by N and C metabolites. Plant J  3, 315-324

Foyer, Lefebvre, Provot, Vincentz, Vaucheret (1993) Modulation of nitrogen and carbon metabolism in transformed Nicotiana plumbaginifolia mutant E23 lines expressing either increased or decreased nitrate reductase activity. Aspects of Applied Biology  34, 137-145

Tourneur,  Jouanin, Vaucheret (1993) Over-expression of acetolactate synthase confers resistance to valine in transgenic tobacc. Plant Science 88, 159-168

Hamza, Camilleri, Pollien, Vaucheret, Bourgin, Chupeau (1993) Selection for spontaneous tomato haploids using a conditional lethal marker. Theor Appl Genet 86, 657-664

Vaucheret (1993) Identification of a general silencer for 19S and 35S promoter in transgenic tobacco: 90 pb of homology in the promoter sequences are sufficient for trans-inactivation. C R Acad Sci Paris, Life Sciences 316, 1471-1483

Vaucheret, Marion-Poll, Meyer, Faure, Marin, Caboche ( 1992)  Interest and limits of reporter genes utilization for the analysis of the transcriptional regulation of nitrate reductase. Mol Gen Genet 235, 259-268

Vermeulen, Vaucheret, Pautot, Chupeau ( 1992) Agrobacterium mediated transfer of a mutant Arabidopsis  acetolactate synthase gene confer resistance to chlorsulfuron in cichory (Cichorium intibus L.). Plant Cell Reports 11, 243-247

Vaucheret, Kronenberger, Lépingle, Vilaine, Boutin, Caboche (1992) Inhibition of tobacco nitrite reductase activity by expression of antisense RNA. Plant J  2, 559-569

Famelaer, Negrutiu, Mouras, Vaucheret, Jacobs (1990) Assymetric hybridization in Nicotiana  by «gamma fusion» and progeny analysis of self-fertile hybrids. Theor Appl Genet 79, 513-520

Vaucheret, Chabaud, Kronenberger, Caboche (1990) Functionnal complementation of tobacco and Nicotiana plumbaginifolia  nitrate reductase deficient mutants by tranformation with the wild type alleles of the tobacco structural genes. Mol Gen Genet 220, 468-474

Pouteau, Chérel, Vaucheret, Caboche (1989) Nitrate reductase mRNA regulation in Nicotiana plumbaginifolia  nitrate reductase deficient mutants. Plant Cell 1, 1111-1120

Vaucheret, Kronenberger, Rouzé, Caboche (1989) Complete nucleotide sequence of the two tobacco homeologous nitrate reductase genes. Plant Mol Biol 12, 597-600

Vaucheret, Vincentz, Kronenberger, Caboche, Rouzé (1989) Molecular cloning and characterization of the two homeologous genes coding for nitrate reductase in tobacco. Mol Gen Genet 216, 10-15

Galangau, Chérel, Rouzé, Vaucheret, Vedele, Vincentz, Caboche (1988) Nitrate reductase expression in tobacco and tomato. Curr Top Plant Biochem Physiol 7, 26-34

Calza, Huttner, Vincentz, Rouzé, Galangau, Vaucheret, Chérel, Meyer, Kronenberger, Caboche (1987) Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductase from higher plants. Mol Gen Genet 209, 552-562

Vaucheret, Signon, Le Bras, Garel (1987) Mechanism of renaturation of a large protein, asparto-kinase-homoserine deshydrogenase. Biochemistry 26, 2785-2790




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