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Meiosis and recombination
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Keywords :Arabidopsis thaliana - Physcomitrella patens- meiosis - meiotic recombination - mitotic recombination- mismatch repair - gene targeting - mutants

Doctoral school affiliation : ED 145 Sciences du végétal
 
Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 7
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19


Meiosis and recombination méiotic
Group leader
Mathilde Grelon
Research Scientist

Raphaël Mercier
Senior Scientist

Eric Jenczewski
Research Scientist

Nathalie Vrielynck
Engineer


Aurélie Chambon
Technician

Laurie Grandont
PhD student

Laurence Cromer
Engineer
PhD student
from 01/11/09 to 31/10/12


Marina Tagliaro Jahns
PhD student
Contract INRA (CJS)
from 01/10/10 to 30/09/13

Wayne Crismani
Post-doc
from 06/07/09 to 04/07/12

Virginie Portemer
Post-doc
15/09/10 au 14/09/13

Aline Epert
Assistant Engineer
ANR GENETOP
from 08/11/10 to 07/11/12

Sarah Bellalou
Engineer
from 01/11/11 to 31/08/12

Chloé Girard
PhD student
Contract young scientist INRA (CJS)
from 1/10/11 to 30/09/14

Afef Lemhamdi
Assistant Engineer
project ERC MeioSight
from 1/01/12 to 31/12/13

Christine Horlow
Research Scientist

Christine Mézard
Senior Scientist

Liudmila Chelysheva
Engineer

Sylvie Jolivet
Technician

Daniel Vezon
Assistant Engineer

Nicole Froger
Technician

Laurène Giraut
Contract

Hossein Khademian
PhD student


Lina Maloukh
Post-doct INRA/DGAP
from 01/11/10 to 31/10/12

Marta Cifuentes
Post-doc

from 01/08/11 to 31/07/12

 

Andrew Lloyd
post doc (ANR)
from 01/07/2011 to 30/06/2013.

Charlotte Renne
Engineer
contract of research RIJK WAAN
from 01/10/11 to 30/09/13

Julien Mazel
Engineer
project ERC MeioSight
from 1/01/12 to 31/12/13

 

 

 

Somatic Recombination

Fabien Nogué
Research Scientist

Anouchka Guyon-Debast
Assistant Engineer

Cécile Collonnier
Research Scientist

Florence Charlot
Assistant Engineer

 

Summary :

 

Main Results :
 

Selected Publications :


Marimuthu MP, Jolivet S, Ravi M, Pereira L, Davda JN, Cromer L, Wang L, Nogué F, Chan SW, Siddiqi I, Mercier R.
Science. 2011 Feb 18;331(6019):876.

d'Erfurth I, Jolivet S, Froger N, Catrice O, Novatchkova M, Mercier R. Turning meiosis into mitosis. PLoS Biol. 2009 Jun 9;7(6):e1000124.

Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly mutant lines.
Curtis MJ, Belcram K, Bollmann SR, Tominey CM, Hoffman PD, Mercier R, Hays JB.
Planta. 2009 Mar;229(4):731-45.

S. Nicolas, M. Leflon,^ H. Monod, F. Eber, O. Coriton, V. Huteau, A.-M. Chèvre et *E. Jenczewski* (2009) *Genetic regulation of meiotic crossovers between related genomes in /Brassica napus/ haploids and hybrids. */The Plant Cell/ 21: 373-385**
De Muyt A, Pereira L, Vezon D, Chelysheva L, Gendrot G, Chambon A, Lainé-Choinard S, Pelletier G, Mercier R, Nogué F, Grelon M. A high throughput genetic screen identifies new early meiotic recombination functions in Arabidopsis thaliana. PLoS Genet. 2009 Sep;5(9):e1000654. Epub 2009 Sep 18.
Liénard D, Nogué F. Physcomitrella patens: a non-vascular plant for recombinant protein production. Methods Mol Biol. 2009;483:135-44.

Alboresi.A, Caffarri.S, Nogue.F, Bassi.R, Morosinotto.T. (2008) In silico and biochemical analysis of Physcomitrella patens photosynthetic antenna: identification of subunits which evolved upon land adaptation. PLoS ONE 3(4) e2033.

Higgins JD, Vignard J, Mercier R, Pugh AG, Franklin FC, Jones GH.. AtMSH5 partners (2008) AtMSH4 in the class I meiotic crossover pathway in Arabidopsis thaliana, but is not required for synapsis. Plant J 55(1) 28-39.

Lienard D, Durambur G, Kiefer-Meyer MC, Nogue F, Menu-Bouaouiche L, Charlot F, Gomord V, Lassalles JP. Water transport by aquaporins in the extant plant Physcomitrella patens.
Plant Physiol.(2008) Jan 9

Lohmiller, L. D. De Muyt, A. Howard, B. Offenberg, H. H. Heyting, C. Grelon, M. Anderson, L. K. (2008) Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato. Chromosoma 117(3) 277-88

Macaisne N, Novatchkova M, Peirera L, Vezon D, Jolivet S, Froger N, Chelysheva L, Grelon M, Mercier R. SHOC1, an XPF endonuclease-related protein, is essential for the formation of class I meiotic crossovers. Curr Biol. (2008) Sep 23;18(18):1432-7.

Mercier R, Grelon M. Meiosis in plants: ten years of gene discovery. Cytogenet Genome Res. (2008);120(3-4):281-90.

Nicolas S.D, Leflon M, Liu Z, Eber F, Chelysheva L, Coriton O, Chevre A.M, Jenczewski E. (2008) Chromosome 'speed dating' during meiosis of polyploid Brassica hybrids and haploids. Cytogenet Genome Res 120(3-4) 331-8.

Pouilly N, Delourme R, Alix K, Jenczewski E. (2008) Repetitive sequence-derived markers tag centromeres and telomeres and provide insights into chromosome evolution in Brassica napus. Chromosome Res 16(5) 683-700.

Salmon A, Clotault J, Jenczewski E, Chable V, Manzanares-Dauleux M. (2008) Brassica oleracea displays a high level of DNA-methylation polymorphism. Plant Science 174 61-70

De Muyt A., Vezon D., Gendrot G., Gallois J.-L., Stevens R., Grelon M. (2007) AtPRD1 is required for meiotic double strand break formation in Arabidopsis thaliana. EMBO J. 2007 Sep 19; 26(18):4126-37

Drouaud J, Mercier R, Chelysheva L, Bérard A, Falque M, Martin O, Zanni V, Brunel D, Mézard C. Sex-specific crossover distributions and variations in interference level along Arabidopsis thaliana chromosome 4.
PLoS Genet. 2007 Jun;3(6):e106. Epub 2007 May 15.

Falque M, Mercier R, Mézard C, de Vienne D, Martin OC. Patterns of recombination and MLH1 foci density along mouse chromosomes: modeling effects of interference and obligate chiasma. Genetics. 2007 Jul;176(3):1453-67. Epub 2007 May 4.

Mezard, C., J. Vignard, J. Drouaud and R. Mercier (2007) The road to crossovers: plants have their say. Trends Genet 23(2): 91-9.

Trouiller B, Charlot F, Choinard S, Schaefer DG, Nogué F. Comparison of gene targeting efficiencies in two mosses suggests that it is a conserved feature of Bryophyte transformation. Biotechnol Lett. 2007 Oct;29(10):1591-8. Epub 2007 Jun 13.

Vignard J, Siwiec T, Chelysheva L, Vrielynck N, Gonord F, Armstrong SJ, Schlögelhofer P, Mercier R. The interplay of RecA-related proteins and the MND1-HOP2 complex during meiosis in Arabidopsis thaliana. PLoS Genet. 2007 Oct;3(10):1894-906. Epub 2007 Aug 30.

A. M. Chèvre, K. Adamczyk, F. Eber, V. Huteau, O. Coriton, J.C. Letanneur, C. Laredo, E. Jenczewski et H. Monod (2007) Modelling gene flow between oilseed rape and wild radish. I. Evolution of chromosome structure. Theoretical & Applied Genetics. 114:209-221 (pdf)

Chelysheva L, Gendrot G, Vezon D, Doutriaux MP, Mercier R, Grelon M. Zip4/Spo22 is required for class I CO formation but not for synapsis completion in Arabidopsis thaliana. PLoS Genet. 2007 May 25;3(5):e83.

S. Nicolas, G. LeMignon, F. Eber, O. Coriton, H. Monod, V. Clouet, V. Huteau, A. Lostanlen, R. Delourme, B. Chalhoub, C. D. Ryder, A.-M. Chèvre et E. Jenczewski (2007) Homeologous recombination is responsible for most of the rearrangements that occur during meiosis of Brassica napus haploids. Genetics. 175: 487 503(StephGenetics2007 (pdf )

Mézard C. Abstract Meiotic recombination hotspots in plants. Biochem Soc Trans. 2006 Aug;34(Pt 4):531-4. Review.

Drouaud J, Camilleri C, Bourguignon PY, Canaguier A, Berard A, Vezon D, Giancola S, Brunel D, Colot V, Prum B, Quesneville H ,Mezard C. Variation in crossing-over rates across chromosome 4 of Arabidopsis thaliana reveals the presence of meiotic recombination "hot spots". Genome Res 16:106-114, (2006).

Jolivet S, Vezon D, Froger N ,Mercier R. Non conservation of the meiotic function of the Ski8/Rec103 homolog in Arabidopsis. Genes Cells 11:615-622, (2006).

Kerzendorfer C, Vignard J, Pedrosa-Harand A, Siwiec T, Akimcheva S, Jolivet S, Sablowski R, Armstrong S, Schweizer D, Mercier R ,Schlogelhofer P. The Arabidopsis thaliana MND1 homologue plays a key role in meiotic homologous pairing, synapsis and recombination. J Cell Sci 119:2486-2496, (2006).

Kopecny D, Tarkowski P, Majira A, Bouchez-Mahiout I, Nogue F, Laurière M, Sandberg G, Laloue M ,Houba-Herin N. Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene. Plant Science 171:114-122, (2006).

M. Leflon, F. Eber, J.C. Letanneur, L. Chelysheva, O. Coriton, V. Huteau, B. Chalhoub, R. Delourme, C. D. Ryder, G Barker, E. Jenczewski and A.M. Chèvre (2006) Pairing and recombination at meiosis of Brassica rapa (AA) x Brassica napus (AACC) hybrids. Theoretical and Applied Genetics 113(8):1467-80. (pdf)

Prosperi JM, Jenczewski E, Angevain M, Ronfort J. Morphologic and agronomic diversity of wild genetic resources of Medicago sativa L. collected in Spain. Genetic Resources and Crop Evolution 53: 843-856, (2006).

Trouiller B, Schaefer DG, Charlot F ,Nogue F. MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens. Nucleic Acids Res 34:232-242, (2006).

Z. Liu, K. Adamczyk, M. Manzanares-Dauleux, F. Eber, M.-O. Lucas, R. Delourme, A.-M. Chèvre et E. Jenczewski (2006) Mapping PrBn and Other Quantitative Trait Loci Responsible for the Control of Homoeologous Chromosome Pairing in Oilseed Rape (Brassica napus L.) Haploids. Genetics. 174(3):1583-96 (LiuGenetics2006 (pdf)

Albertin W, Brabant P, Catrive O, Eber F, Jenczewski E, Chevre AM, Thiellement H. Autopolyploidy in cabbage (Brassica oleracea L.) does not alter significantly the proteomes of green tissues. Proteomics 5: 2131-2139, (2005).

Chelysheva L, Diallo S, Vezon D, Gendrot G, Vrielynck N, Belcram K, Rocques N, Marquez-Lema A, Bhatt A, Horlow C, Mercier R, Mezard C. AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis. J Cell Sci, 118(Pt 20):4621-32. (2005).

Lago C, Clerici E, Dreni L, Horlow C, Caporali E, Colombo L, Kater MM. The Arabidopsis TFIID factor AtFAF6 controls pollen tube growth. Dev Biol. 285(1):91-100,(2005)

Mercier R, Jolivet S, Vezon D, Huppe E, Chelysheva L., Giovanni M., Nogue F., Doutriaux MP, Horlow C, Grelon M Mezard C. Two meiotic crossover classes cohabit in Arabidopsis : one is dependent on MER3, whereas the other one is not. Curr Biol 15 : 692-701, (2005).

Prieto JL, Pouilly N, Jenczewski E, Deragon JM, Chèvre AM. Development of crop-specific transposable element (SINE) markers to study gene flow from oilseed rape to wild radish. Theoretical and Applied Genetics 111(3):446-55, 2005
Sanchez-Moran E, Mercier R, Higgins JD, Armstrong SJ, Jones GH, Franklin FC. A strategy to investigate the plant meiotic proteome, Cytogenet Genome Res, 109 (1-3) p181-9, (2005).

Vincent P, Chua M, Nogue F, Fairbrother A, Mekheel H, Xu Y, Allen N, Bibikova T, Gilroy S, Bankaitis V. Sec14p-nodulin-domain phosphatidylinositol transfer protein polarizes membrane growth of Arabidopsis root hairs . J Cell Biol. 168: 801-812, (2005)

Bohme K, Li Y, Charlot F, Grierson C, Marrocco K, Okada K, Laloue M, Nogue F. The Arabidopsis COW1 gene encodes a phosphatidylinositol transfer protein essential for root hair tip growth. Plant J. 40: 686-698, (2004)

E. Jenczewski et K. Alix. (2004) From diploids to allopolyploids: the emergence of pairing control genes. Critical Reviews in Plant Sciences 23(1): 21-45

Stevens, R., Grelon, M., Vezon, D., Domenichini, S., Bergounioux, C. (2004) A cdc45 homologue in Arabidopsis thaliana has a role in meiosis as shown by RNAi induced gene silencing. Plant Cell., Jan;16(1):99-113

E. Jenczewski, J. Ronfort et A.M. Chèvre (2003) Crop-to-wild gene flow, introgression and possible fitness effects of transgenes. Environmental Biosafety Research 2, 9-24.

E. Jenczewski, F. Eber, A. Grimaud, S. Huet, M.O. Lucas, H. Monod et A.M. Chèvre (2003) PrBn: a major gene controlling homoeologous pairing in oilseed rape (Brassica napus) dihaploids. Genetics 164: 645-654. (pdf)

Grelon, M., Gendrot, G., Vezon, D., Pelletier, G. (2003) The Arabidopsis MEI1 gene encodes a protein with five BRCT domains that is involved in meiosis-specific DNA repair events independent of SPO11-induced DSBs. Plant J., 35 : 465-475

Mercier R, Armstrong SJ, Horlow C, Jackson NP, Makaroff CA, Vezon D, Pelletier G, Jones GH, Franklin FCH: The meiotic protein SWI1 is required for axial element formation and recombination initiation in Arabidopsis. Development 130: 3309-3318 (2003).

Peciña A., Smith K. N., Mézard C., Murahami H., Ohta K., and Nicolas A. (2002). Targeted stimulation of meiotic recombinaison. Cell 111, 173-184

Horlow C et Doutriaux MP (2002) Les mécanismes moléculaires de la méiose chez les plantes. Médecine Science (Sous Presse).

Brun F, Gonneau M, Doutriaux MP, Laloue M, Nogué F (2001). Cloning of the PpMSH-2 cDNA of Physcomitrella patens, a moss in which gene targeting by homologous recombination occurs at high frequency. Biochimie 83, 1-6.

Grelon M, Vezon D, Gendrot G, Pelletier G (2001). AtSPO11-1 is neccessary for efficient meiotic recombination in plants. EMBO J. Feb 1;20(3), 589-600.

Mercier R, Grelon M, Vezon D, Horlow C, Pelletier G (2001a). How to characterize meiotic functions in plants? Biochimie. Nov-Dec;83(11-12), 1023-8.

Mercier R, Vezon D, Bullier E, Motamayor JC, Sellier A, Lefèvre F, Pelletier G and. Horlow C (2001b) SWITCH 1 (SWI1) : a novel protein required for the establishment of sister chromatid cohesion and for bivalent formation at meiosis. Genes and Dev 15, 1859-71.

Couteau F, Belzile F, Horlow C, Grandjean O, Vezon D, and Doutriaux MP (1999). Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of Arabidopsis. Plant Cell, 11, 9, 1623-1634.

Further Readings:

 

 

  

 


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