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Epigénétique et petits ARNs
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Mots-clés :Arabidopsis thaliana - Mutant - Chromatine - Methylation - Interference ARN - Developpement - siRNA - miRNA - Virus

Ecole(s) doctorale(s) de rattachement :

Contacts :

Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 2
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

tél : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19

 

Responsable
Hervé Vaucheret
Directeur de recherche

Co-responsable
Taline Elmayan
Chargé de recherche

Catherine Albertini
Ingénieur de recherche

 

 

Nathalie Bouteiller
Technicien

Jean Sébastien Parent
Post-doc
du 01/12/10 au 30/11/12

Wael Wahib Mahmoud
Master 2
du 3/01/12 au 29/06/12

 

Anciens membres
de l'équipe

Co-responsable
Allison Mallory

Chargé de recherche

Ivan Le Masson
Ingénieur de recherche

Emilio Martinez
Post-doc
du 15/05/09 au 14/05/12

Nahid Shamandi
Doctorant
du 01/10/10 au 30/09/13

Emilie Elvira-Matelot
Post-doc
du 03/01/11 au 31/12/13

 

Baptiste Saudemont
post-doc
du 15/11/11 au 15/07/13


Résumé :

TGS du gene GUS Les eucaryotes régulent l’expression génique grâce à des mécanismes d'inactivation épigénétique faisant intervenir des petits ARN de 21-24 nucléotides chlorose due a la PTGS de la nitrite reductase (siRNAs et miRNAs) conduisant soit à un blocage de la transcription (transcriptional gene silencing: TGS), soit à une dégradation des ARN (post-transcriptional gene silencing: PTGS), soit à une répression de la traduction. Les cibles de ces processus sont des éléments exogènes (virus, bactéries) ou endogènes (gènes, transposons, hétérochromatine). Une approche de génétique moleculaire a été entreprise pour identifier les gènes cellulaires contrôlant ces processus et comprendre les mécanismes sous-jacents.

 


Résultats marquants :

chlorose due a la PTGS de la nitrite reductase Nos travaux ont mis en évidence plusieurs protéines cellulaires participant à différents réseaux de silencing : TGS et PTGS des transgènes, atténuation de l’infection virale, régulation post-transcriptionnelle de gènes endogènes par des microRNAs (miRNAs) ou des trans-acting siRNAs (ta-siRNAs), contrôle transcriptionnel des éléments répétés du génome. Outre leur rôle dans la régulation du développement, certains miRNAs et ta-siRNAs participent à des boucles de rétro-action permettant de maintenir l’homéostasie de ces réseaux complexes de régulation.




Publications représentatives :

Vaucheret, H., and Chupeau, Y. (2011). Ingested plant miRNAs regulate gene expression in animals. Cell Research. doi:10.1038/cr.2011.

Martinez de Alba, A.E., Jauvion, V., Mallory, A.C., Bouteiller, N., and Vaucheret, H. (2011). The miRNA pathway limits AGO1 availability during siRNA-mediated PTGS defense against exogenous RNA. Nucleic Acids Research. doi: 10.1093/nar/gkr590 (PubMed)

Pelissier, T., Clavel, M., Chaparro, C., Pouch-Pelissier, M.N., Vaucheret, H., and Deragon, J.M. (2011). Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis. RNA 17, 1502-1510. (PubMed)

Wang, X.B., Jovel, J., Udomporn, P., Wang, Y., Wu, Q., Li, W.X., Gasciolli, V., Vaucheret, H., and Ding, S.W. (2011). The 21-nucleotide, but not 22-nucleotide, viral secondary small interfering RNAs direct potent antiviral defense by two cooperative argonautes in Arabidopsis thaliana. Plant Cell 23, 1625-1638. (PubMed)

Hirsch, J., Misson, J., Crisp, P.A., David, P., Bayle, V., Estavillo, G.M., Javot, H., Chiarenza, S., Mallory, A.C., Maizel, A., Declerck, M., Pogson, B.J., Vaucheret, H., Crespi, M., Desnos, T., Thibaud, M.C., Nussaume, L., and Marin, E. (2011). A novel fry1 allele reveals the existence of a mutant phenotype unrelated to 5'->3' exoribonuclease (XRN) activities in Arabidopsis thaliana roots. PLoS One 6, e16724. (pdf)

Jauvion, V., Mallory, A.C., and Vaucheret, H. (2011) Deciphering post-transcriptional gene silencing pathways through genetic screens. In RNAi technology. Gaur, R.K., Gafni, Y., Sharma, P., and Gupta, V.K. editors, CRC press. Science publishers, p17-46

Vaucheret, H. (2011) 2001, l’odyssée des petits ARN. Biofutur 30, 32-34

Form, Function, and Regulation of ARGONAUTE Proteins. Mallory A, Vaucheret H. Plant Cell. 2010 Dec 23. [Epub ahead of print]

The conserved RNA trafficking proteins HPR1 and TEX1 are involved in the production of endogenous and exogenous small interfering RNA in Arabidopsis. Jauvion V, Elmayan T, Vaucheret H. Plant Cell. 2010 Aug;22(8):2697-709. Epub 2010 Aug 26.

siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. Yu B, Bi L, Zhai J, Agarwal M, Li S, Wu Q, Ding SW, Meyers BC, Vaucheret H, Chen X. Nucleic Acids Res. 2010 Sep 1;38(17):5844-5850. Epub 2010 May 6. PMID: 20448024 [PubMed - as supplied by publisher]Free PMC ArticleFree text

miR390, Arabidopsis TAS3 tasiRNAs, and their AUXIN RESPONSE FACTOR targets define an autoregulatory network quantitatively regulating lateral root growth. Marin E, Jouannet V, Herz A, Lokerse AS, Weijers D, Vaucheret H, Nussaume L, Crespi MD, Maizel A. Plant Cell. 2010 Apr;22(4):1104-17. Epub 2010 Apr 2. PMID: 20363771 [PubMed - indexed for MEDLINE]

Mallory, A.C., Hinze, A. Tucker, M., Bouche, N., Gasciolli, V., Elmayan, T., Lauressergues, D., Jauvion, V., Vaucheret, H. and Laux, T.1 (2009) Redundant and specific roles of the ARGONAUTE proteins AGO1 and ZLL in development and small RNA-directed gene silencing. PloS Genetics 5, e1000646. doi:10.1371/journal.pgen.1000646 (PubMed)

Vaucheret H (2009) AGO1 Homeostasis Involves Differential Production of 21-nt and 22-nt miR168 Species by MIR168a and MIR168b. PLoS ONE 4(7):e6442. doi:10.1371/journal.pone.0006442 (PubMed)

Mallory AC, Vaucheret H (2009) ARGONAUTE 1 homeostasis invokes the coordinate action of the microRNA and siRNA pathways. EMBO Rep 10: 521-526.(PubMed)

Elmayan T, Adenot X, Gissot L, Lauressergues D, Gy I, et al. (2009) A neomorphic sgs3 allele stabilizing miRNA cleavage products reveals that SGS3 acts as a homodimer. FEBS J 276: 835-844.(PubMed)

Ben Amor B, Wirth S, Merchan F, Laporte P, d'Aubenton-Carafa Y, et al. (2009) Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses. Genome Res 19: 57-69.( PubMed)

Meyers BC, Axtell MJ, Bartel B, Bartel DP, Baulcombe D, et al. (2008) Criteria for annotation of plant MicroRNAs. Plant Cell 20: 3186-3190.( PubMed)

Mallory, A.C., Elmayan, T. and Vaucheret, H. (2008) MicroRNA maturation and action--the expanding roles of ARGONAUTEs. Curr Opin Plant Biol, 11, 560-566.( PubMed)

Pouch-Pelissier, M.N., Pelissier, T., Elmayan, T., Vaucheret, H., Boko, D., Jantsch, M.F., and Deragon, J.M. (2008). SINE RNA induces severe developmental defects in Arabidopsis thaliana and interacts with HYL1 (DRB1), a key member of the DCL1 complex. PLoS Genet 4, e1000( PubMed)

Vaucheret, H. (2008). Plant ARGONAUTES. Trends Plant Sci 13, 350-358(PubMed)

Mallory, A.C., and Bouche, N. (2008). MicroRNA-directed regulation: to cleave or not to cleave. Trends Plant Sci 13, 359-367.(PubMed)

Daxinger L, Hunter B, Sheikh M, Jauvion V, Gasciolli V, Vaucheret H, Matzke M, Furner I (2008) Unexpected silencing effects from T-DNA tags in Arabidopsis. Trends in Plant Science 13, 4-7 (PubMed)

II Gy, V Gasciolli, D Lauressergues, J-B Morel, J Gombert, F Proux, C Proux, H Vaucheret, and AC. Mallory (2007) Plant Cell Arabidopsis FIERY1, XRN2, and XRN3 Are Endogenous RNA Silencing Suppressors 10.1105/tpc.107.055319 (PubMed) and free download

Mourrain P, van Blockland R, Kooter J, Vaucheret H (2007) A single transgene locus triggers both transcriptional and post-transcriptional silencing through double-stranded RNA production. Planta 225, 365-79 (PubMed)

Rajagopalan R, Vaucheret H, Trejo J, Bartel DP (2006) A diverse and evolutionarily fluid set of miRNAs in Arabidopsis thaliana. Genes Dev, 20, 3407-25 (PubMed)

Bouché, Lauressergues, Gasciolli,Vaucheret (2006) An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs. EMBO Journal, 25, 3347-56 (PubMed)

Mallory, Vaucheret (2006) Functions of plant microRNAs and related small RNAs. Nature Genetics 38, S31-S36 (PubMed)

Adenot, Elmayan, Lauressergues, Boutet, Bouché, Gasciolli, Vaucheret (2006) DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7. Current Biology 16, 927-932 (PubMed)

Vaucheret (2006) Posttranscriptional small RNA pathways in plants: mechanisms and regulations. Genes & Development 20, 759-771 (PubMed)

Vaucheret, Mallory, Bartel (2006) AGO1 homeostasis entails co-expression of MIR168 and AGO1 and preferential stabilization of miR168 by AGO1. Molecular Cell 22, 129-136 (PubMed)

Elmayan, Proux, Vaucheret (2005) Arabidopsis RPA2: a genetic link between transcriptional gene silencing, DNA repair and DNA replication. Curr Biol 15, 1919-1925 (PubMed)

Vaucheret (2005) MicroRNA-dependent trans-acting siRNA production. Science STKE 2005, pe43 (PubMed)

Gasciolli*, Mallory*, Bartel, Vaucheret (2005) Partially redundant functions of Arabidopsis Dicer-like enzymes and a role for DCL4 in producing trans-acting siRNAs. Curr Biol 15, 1494-1500 (PubMed)

Vaucheret (2005) RNA polymerase IV and transcriptional silencing. Nat Gen 37, 659-660 (PubMed)

Park, Wu, Gonzalez-Sulser, Vaucheret, Poethig (2005) Nuclear processing and export of miRNAs in Arabidopsis. Proc. Natl. Acad. Sci. USA 102, 3691-3696. (PubMed)

Rocha, Sheikh, Melchiorre, Fagard, Boutet, Loach, Moffatt, Wagner, Vaucheret, Furner (2005) The Arabidopsis HOG1 gene codes for a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing. Plant Cell 17, 404-415 (PubMed)

Muangsan, Beclin, Vaucheret, Robertson (2004) Genetic requirements for geminivirus-mediated VIGS of endogenous genes define a new branch in the plant silencing pathway. Plant J 38, 1004-1014 (PubMed)

Vazquez*, Vaucheret*, Rajagopalan, Lepers, Gasciolli, Mallory, Hilbert, Bartel, Crété (2004) Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs. Mol Cell 16, 69-79 (PubMed)

Mallory, Vaucheret (2004) MicroRNAs: something important between the genes. Curr Opi Plant Biol 7, 120-125 (PubMed)

Vazquez, Gasciolli, Crété, Vaucheret (2004) The nuclear double-stranded RNA-binding protein HYL1 is required for proper microRNA accumulation and plant development but not for post-transcriptional transgene silencing. Curr Biol 14, 346-351 (PubMed)

Probst, Fagard, Proux, Mourrain, Boutet, Murfett, Furner, Vaucheret, Mittelstein-Scheid (2004) Arabidopsis histone deacetylase HDA6 is required for maintenance of transcriptional gene silencing and determines DNA methylation and nuclear organization of rDNA repeats. PlantCell 16, 1021-1034 (PubMed)

Vaucheret, Vazquez, Crété, Bartel (2004)The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev 18, 1187-97 (PubMed)

Boutet, Vazquez, Liu, Beclin, Fagard, Gratias, Morel, Crété, Chen, Vaucheret(2003) Arabidopsis HEN1 : a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Curr Biol 13, 843-848 (PubMed)

Morel, Godon, Mourrain, Béclin, Boutet, Feuerbach, Proux, Vaucheret (2002) Fertile hypomorphic ARGONAUTE (ago1) mutants impaired in post-transcriptional gene silencing and virus resistance. Plant Cell 14, 629-639 (PubMed)

Béclin, Boutet, Waterhouse, Vaucheret (2002) A branched pathway for transgene-induced RNA silencing in plants. Curr Biol 12, 684-688 (PubMed)

Mlotshwa, Voinnet, Mette, Matzke, Vaucheret, Ding, Pruss, Vance (2002) RNA silencing and the mobile signal. Plant Cell S289-S301(PubMed)

Vaucheret, Fagard (2001) Transcriptional gene silencing in plants: targets, inducers and regulators. Trends in Genet 17, 29-35 (PubMed)

Mallory, Ely, Smith, Marathe, Anandalakshmi, Fagard, Vaucheret, Pruss, Bowman, Vance (2001) HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal . Plant Cell 13, 571-583 (PubMed)

Vance, Vaucheret (2001) RNA silencing in plants - Defense and counterdefense. Science 292, 2277-2280 (PubMed)

Mourrain, Béclin, Elmayan, Feuerbach, Godon, Morel, Jouette, Lacombe, Nikic, Picault, Rémoué, Sanial, Vo, Vaucheret. (2000) Arabidopsis SGS2 and SGS3 genes required for post-transcriptional gene silencing and virus resistance. Cell 101, 533-542 (PubMed)

Fagard, Boutet, Morel, Bellini, Vaucheret (2000) AGO1, QDE-2 and RDE-1 encode related proteins required for post-transcriptional gene silencing in plants, quelling in fungi and RNA interference in animals. Proc. Natl. Acad. Sci. USA 97, 11650-11654 (PubMed)

Morel, Mourrain, Béclin, Vaucheret (2000) DNA methylation and chromatin structure affect transcriptional and post- transcriptional transgene silencing in Arabidopsis. Curr Biol 10, 1591-1594 (PubMed)

Palauqui, Vaucheret (1998) Transgenes are dispensable for the RNA degradation step of co-suppression. Proc Natl Acad Sci USA 95, 9675-9680 (PubMed)

Elmayan, Balzergue, Béon, Bourdon, Daubremet, Guénet, Mourrain, Palauqui, Vernhettes, Vialle, Wostrikoff,  Vaucheret (1998) Arabidopsis mutants impaired in cosuppression. Plant Cell 10, 1747-1758 (PubMed)

Vaucheret, Nussaume, Palauqui, Quilléré, Elmayan (1997) A transcriptionally active state is required for post-transcriptional silencing (co-suppression) of nitrate reductase host genes and transgenes. Plant Cell 9, 1495-1504 (PubMed)

Palauqui, Elmayan, Pollien, Vaucheret (1997) Systemic Acquired Silencing: Transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J 16, 4738-4745 (PubMed)

 

  

 


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